Therapy against cancer remains a daunting issue for human health, despite remarkable innovations in many areas of pathology. In situ biosynthesized nanoclusters bestow a novel remedy for carcinogenic cell imaging. Exosomes have received special attention as an efficient tool for the diagnosis of various diseases, including cancers. All types of cells (healthy or diseased) generate exosomes, making them significantly unique for relevant disease diagnosis and treatment. In this contribution, we exploit the possibility of utilizing the exosomes to facilitate chemotherapeutics, viz. the combination of doxorubicin (Dox) and biosynthesized silver nanoclusters in cancer cells. Our study showed a new facile way for bioimaging of cancer cells using biosynthesized silver-DNA nanoclusters, and thus further targeting cancer cells using the relevant cancer exosomes as drug delivery cargo. After isolating exosomes from neoplastic cells, i.e. HeLa, loaded with the drug, and treating other neoplastic cells with cargo-loaded isolated exosomes, we found that cargo-loaded isolated exosomes can readily enter into the targeted cancer cells and efficiently kill these neoplastic cells. This raises the possibility of acting as a novel facile modality for target cancer theranostics with high efficiency and biocompability.

尽管在许多病理学领域取得了显着的创新，但癌症治疗仍然是人类健康的一个令人生畏的问题。原位生物合成的纳米团簇为致癌细胞成像提供了一种新的治疗方法。外泌体作为诊断包括癌症在内的各种疾病的有效工具而受到特别关注。所有类型的细胞（健康的或患病的）都会产生外泌体，这使得它们在相关疾病的诊断和治疗中具有显着的独特性。在这项贡献中，我们利用外泌体促进化疗的可能性，即。多柔比星 (Dox) 和癌细胞中生物合成的银纳米簇的组合。我们的研究展示了一种使用生物合成的银-DNA纳米簇对癌细胞进行生物成像的简便新方法，从而使用相关的癌症外泌体作为药物递送货物进一步靶向癌细胞。在从肿瘤细胞中分离出外泌体后，即载有药物的 HeLa，并用装载货物的分离外泌体治疗其他肿瘤细胞，我们发现装载货物的分离外泌体可以很容易地进入靶向癌细胞并有效杀死这些肿瘤细胞。这提高了作为具有高效率和生物相容性的靶向癌症治疗学的新型简便模式的可能性。