Roseburia species are important denizens of the human gut microbiome that ferment complex polysaccharides to butyrate as a terminal fermentation product, which influences human physiology and serves as an energy source for colonocytes. Previous comparative genomics analyses of the genus Roseburia have examined polysaccharide degradation genes. Here, we characterize the core and pangenomes of the genus Roseburia with respect to central carbon and energy metabolism, as well as biosynthesis of amino acids and B vitamins using orthology-based methods, uncovering significant differences among species in their biosynthetic capacities. Variation in gene content among Roseburia species and strains was most significant for cofactor biosynthesis. Unlike all other species of Roseburia that we analysed, Roseburia inulinivorans strains lacked biosynthetic genes for riboflavin or pantothenate but possessed folate biosynthesis genes. Differences in gene content for B vitamin synthesis were matched with differences in putative salvage and synthesis strategies among species. For example, we observed extended biotin salvage capabilities in R. intestinalis strains, which further suggest that B vitamin acquisition strategies may impact fitness in the gut ecosystem. As differences in the functional potential to synthesize components of biomass (e.g. amino acids, vitamins) can drive interspecies interactions, variation in auxotrophies of the Roseburia spp. genomes may influence in vivo gut ecology. This study serves to advance our understanding of the potential metabolic interactions that influence the ecology of Roseburia spp. and, ultimately, may provide a basis for rational strategies to manipulate the abundances of these species.

中文翻译：

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罗斯布里亚属的比较基因组学揭示了可能影响物种间结肠竞争的不同生物合成途径。

罗斯布里亚 属物种是人类肠道微生物组的重要居民，它们将复杂的多糖发酵为丁酸盐作为最终发酵产物，影响人类生理机能并作为结肠细胞的能量来源。先前对Roseburia属的比较基因组学分析已经检查了多糖降解基因。在这里，我们利用基于直系同源的方法，在中心碳和能量代谢以及氨基酸和 B 族维生素的生物合成方面描述了Roseburia属的核心和全基因组罗斯布里亚属物种和菌株之间基因含量的变化对于辅因子生物合成最为显着。与我们分析的所有其他Roseburia物种不同， Roseburia inulinivorans菌株缺乏核黄素或泛酸的生物合成基因，但拥有叶酸生物合成基因。B 族维生素合成基因含量的差异与物种间假定的回收和合成策略的差异相匹配。例如，我们观察到肠杆菌菌株具有延长的生物素回收能力，这进一步表明 B 族维生素获取策略可能会影响肠道生态系统的健康。由于合成生物质成分（例如氨基酸、维生素）的功能潜力的差异可以驱动种间相互作用，罗斯伯利亚属营养缺陷型的变化。基因组可能影响体内肠道生态。这项研究有助于加深我们对影响罗斯伯利亚属生态的潜在代谢相互作用的理解。最终，可能为操纵这些物种的丰度的合理策略提供基础。