DNA analysis of degraded samples and low‐copy number DNA derived from skeletal remains, one of the most challenging forensic tasks, is common in disaster victim identification and genetic analysis of historical materials. Massively parallel sequencing (MPS) is a useful technique for STR analysis that enables the sequencing of smaller amplicons compared with conventional capillary electrophoresis (CE), which is valuable for the analysis of degraded DNA. In this study, 92 samples of human skeletal remains (70+ years postmortem) were tested using an in‐house MPS‐STR system designed for the analysis of degraded DNA. Multiple intrinsic factors of DNA from skeletal remains that affect STR typing were assessed. The recovery of STR alleles was influenced more by DNA input amount for amplification rather than DNA degradation, which may be attributed from the high quantity and quality of libraries prepared for MPS run. In addition, the higher success rate of STR typing was achieved using the MPS‐STR system compared with a commercial CE‐STR system by providing smaller sized fragments for amplification. The results can provide constructive information for the analysis of degraded sample, and this MPS‐STR system will contribute in forensic application with regard to skeletal remain sample investigation.

中文翻译：

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通过内部MPS-STR面板改进了从人体骨骼残留物中降解的DNA的STR分析。

降解样品的DNA分析和低拷贝数从骨骼残留物中提取的DNA是最具挑战性的法医任务之一，在灾难受害者识别和历史材料的遗传分析中很常见。大规模并行测序（MPS）是用于STR分析的有用技术，与常规毛细管电泳（CE）相比，它可以对较小的扩增子进行测序，这对于分析降解的DNA具有重要意义。在这项研究中，使用为分析降解的DNA设计的内部MPS-STR系统测试了92个人体骨骼样本（死后70多年）。评估了骨骼残骸中影响STR分型的DNA的多个内在因素。STR等位基因的恢复受DNA扩增量（而不是DNA降解）的影响更大，这可能归因于为MPS运行准备的大量和高质量的库。此外，与商用CE-STR系统相比，使用MPS-STR系统通过提供较小的片段进行扩增，可实现STR分型的更高成功率。结果可以为降解样品的分析提供建设性的信息，该MPS-STR系统将在骨骼残留样品研究的法医应用中做出贡献。