Wolbachia is a genus containing obligate, intracellular endosymbionts with arthropod and nematode hosts. Numerous studies have identified differentially expressed transcripts in Wolbachia endosymbionts that potentially inform the biological interplay between these endosymbionts and their hosts, albeit with discordant results. Here, we re-analyze previously published Wolbachia RNA-Seq transcriptomics data sets using a single workflow consisting of the most up-to-date algorithms and techniques, with the aim of identifying trends or patterns in the pan-Wolbachia transcriptional response. We find that data from one of the early studies in filarial nematodes did not allow for robust conclusions about Wolbachia differential expression with these methods, suggesting the original interpretations should be reconsidered. Across datasets analyzed with this unified workflow, there is a general lack of global gene regulation with the exception of a weak transcriptional response resulting in the upregulation of ribosomal proteins in early larval stages. This weak response is observed across diverse Wolbachia strains from both nematode and insect hosts suggesting a potential pan-Wolbachia transcriptional response during host development that diverged more than 700 million years ago.

沃尔巴氏菌属是一种带有节肢动物和线虫宿主的专性细胞内共生菌属。许多研究已经鉴定出在沃尔巴克氏菌内共生体中差异表达的转录本，尽管可能导致不一致的结果，但它们可能提示这些内共生菌与其宿主之间的生物学相互作用。在这里，我们使用由最新算法和技术组成的单一工作流程，重新分析先前发布的沃尔巴克氏菌RNA-Seq转录组学数据集，目的是确定泛沃尔巴克氏菌转录反应的趋势或模式。我们发现来自丝状线虫的一项早期研究的数据不能得出有关沃尔巴克氏菌的可靠结论这些方法的差异表达，建议应重新考虑原始解释。在使用这种统一的工作流程分析的所有数据集中，普遍缺乏全局基因调节，只是转录响应较弱，导致幼虫早期的核糖体蛋白上调。在来自线虫和昆虫寄主的多种沃尔巴氏菌菌株中观察到这种弱应答，表明在七亿多年前发散的寄主发育过程中潜在的泛沃尔巴氏菌转录应答。