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Immuno- and glyco-histochemistry as a tool to evaluate the oregano supplemented feed effects in pig gut.
European Journal of Histochemistry ( IF 2 ) Pub Date : 2020-02-20 , DOI: 10.4081/ejh.2020.3110
Cecilia Dall'Aglio 1 , Francesca Mercati , Valerio Faeti , Gabriele Acuti , Massimo Trabalza Marinucci , Elena De Felice , Federico Maria Tardella , Maria Pia Franciosini , Patrizia Casagrande Proietti , Daniele Catorci , Paolo Stacchini , Augusto Pastorelli , Paola Scocco
Affiliation  

Among oregano properties, its antioxidant and antibacterial effects are particularly interesting. Oregano is also able to induce a higher glycoconjugate production in gut, creating a physical barrier against microorganisms. This study evaluated the effects of adding an aqueous extract of oregano (OAE) to the diet of two homogenous groups of pigs during the finisher phase. The diets were as follows: control commercial diet (CTR group) and CTR diet supplemented (2 g/kg) with OAE (O group). Samples of ileum and caecum from the two groups were examined by conventional histochemistry to analyze complex carbohydrates and by immunohistochemistry to detect Bcl-2 Associate X protein (BAX), an indicator of oxidative stress. Glyco-histochemistry showed significant differences between the two groups. Immunohistochemistry revealed a lower presence of BAX in O group. The OAE supplementation improved the production of glycoconjugates, able to enhance in pig the protection of intestinal mucosa by means of direct and indirect defense actions. The reduced BAX immunostaining observed in O group may be an indicator of enhanced antioxidant action promoted by oregano. The results of this study can be used in further research to identify ways to improve endogenous defence ability, with the aim of reducing antibiotic use and preventing antimicrobial resistance.

中文翻译:

免疫和糖组织化学作为评估牛至补充猪肠饲料效果的工具。

在牛至的性质中,其抗氧化剂和抗菌作用特别令人感兴趣。牛至还能够诱导肠道中更高的糖缀合物产生,从而形成针对微生物的物理屏障。这项研究评估了在肥育阶段将牛至水提取物(OAE)添加到两组同质猪的日粮中的效果。饮食如下:对照商业饮食(CTR组)和补充有OAE的CTR饮食(2 g / kg)(O组)。两组的回肠和盲肠样品通过常规组织化学检查以分析复杂的碳水化合物,并通过免疫组织化学检查以检测Bcl-2关联X蛋白(BAX)(一种氧化应激指标)。糖组化显示两组之间有显着差异。免疫组织化学显示,O组中BAX的含量较低。OAE补充剂改善了糖缀合物的产生,能够通过直接和间接防御作用增强猪对肠粘膜的保护。O组中观察到的BAX免疫染色降低可能是牛至促进抗氧化作用增强的指示。这项研究的结果可用于进一步的研究,以确定提高内源性防御能力的方法,以减少抗生素的使用并预防抗菌素耐药性。O组中观察到的BAX免疫染色降低可能是牛至所促进的抗氧化作用增强的指示。这项研究的结果可用于进一步的研究,以确定提高内源性防御能力的方法,目的是减少抗生素的使用并预防抗菌素耐药性。O组中观察到的BAX免疫染色降低可能是牛至所促进的抗氧化作用增强的指示。这项研究的结果可用于进一步的研究,以确定提高内源性防御能力的方法,目的是减少抗生素的使用并预防抗菌素耐药性。
更新日期:2020-02-20
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