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Challenges and opportunities with CRISPR activation in bacteria for data-driven metabolic engineering.
Current Opinion in Biotechnology ( IF 7.7 ) Pub Date : 2020-06-27 , DOI: 10.1016/j.copbio.2020.04.005
Jason Fontana 1 , David Sparkman-Yager 1 , Jesse G Zalatan 2 , James M Carothers 3
Affiliation  

Creating CRISPR gene activation (CRISPRa) technologies in industrially promising bacteria could be transformative for accelerating data-driven metabolic engineering and strain design. CRISPRa has been widely used in eukaryotes, but applications in bacterial systems have remained limited. Recent work shows that multiple features of bacterial promoters impose stringent requirements on CRISPRa-mediated gene activation. However, by systematically defining rules for effective bacterial CRISPRa sites and developing new approaches for encoding complex functions in engineered guide RNAs, there are now clear routes to generalize synthetic gene regulation in bacteria. When combined with multi-omics data collection and machine learning, the full development of bacterial CRISPRa will dramatically improve the ability to rapidly engineer bacteria for bioproduction through accelerated design-build-test-learn cycles.

中文翻译:

用于数据驱动的代谢工程的细菌中CRISPR激活面临的挑战和机遇。

在工业上有前途的细菌中创建CRISPR基因激活(CRISPRa)技术可能对加速数据驱动的代谢工程和菌株设计具有革命性意义。CRISPRa已被广泛用于真核生物,但在细菌系统中的应用仍然受到限制。最近的工作表明细菌启动子的多种特征对CRISPRa介导的基因激活提出了严格的要求。但是,通过系统地定义有效细菌CRISPRa位点的规则并开发新的方法来编码工程指导RNA中的复杂功能,现在有了明确的途径可以概括细菌中的合成基因调控。当与多组学数据收集和机器学习结合使用时,
更新日期:2020-06-26
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