SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed Cases.,mSystems

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SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed Cases.
mSystems ( IF 6.4 ) Pub Date : 2020-07-21 , DOI: 10.1128/msystems.00614-20
Fuqing Wu _{1,

2,

3} , Jianbo Zhang _{1,

2} , Amy Xiao _{1,

2,

3} , Xiaoqiong Gu _{4,

5} , Wei Lin Lee _{4,

5} , Federica Armas _{4,

5} , Kathryn Kauffman ₆ , William Hanage ₇ , Mariana Matus ₈ , Newsha Ghaeli ₈ , Noriko Endo ₈ , Claire Duvallet ₈ , Mathilde Poyet _{1,

2,

3} , Katya Moniz _{1,

2,

3} , Alex D Washburne ₉ , Timothy B Erickson _{10,

11} , Peter R Chai _{10,

12,

13} , Janelle Thompson _{5,

14,

15} , Eric J Alm _{2,

3,

4,

5,

8,

16,

17}

Affiliation

Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Singapore-MIT Alliance for Research and Technology, National University of Singapore, Singapore.
Campus for Research Excellence and Technological Enterprise, Singapore.
University at Buffalo, The State University of New York, Buffalo, New York, USA.
Center for Communicable Disease Dynamics, Department of Epidemiology, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, USA.
Biobot Analytics, Inc., Cambridge, Massachusetts, USA.
Selva Analytics, LLC, Bozeman, Montana, USA.
Division of Medical Toxicology, Department of Emergency Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Harvard Humanitarian Institute, Cambridge, Massachusetts, USA.
The Fenway Institute, Boston, Massachusetts, USA.
The Koch Institute for Integrated Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Singapore Center for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore.
Asian School of the Environment, Nanyang Technological University, Singapore.
Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.

Wastewater surveillance represents a complementary approach to clinical surveillance to measure the presence and prevalence of emerging infectious diseases like the novel coronavirus SARS-CoV-2. This innovative data source can improve the precision of epidemiological modeling to understand the penetrance of SARS-CoV-2 in specific vulnerable communities. Here, we tested wastewater collected at a major urban treatment facility in Massachusetts and detected SARS-CoV-2 RNA from the N gene at significant titers (57 to 303 copies per ml of sewage) in the period from 18 to 25 March 2020 using RT-qPCR. We validated detection of SARS-CoV-2 by Sanger sequencing the PCR product from the S gene. Viral titers observed were significantly higher than expected based on clinically confirmed cases in Massachusetts as of 25 March. Our approach is scalable and may be useful in modeling the SARS-CoV-2 pandemic and future outbreaks.

中文翻译：

废水中的 SARS-CoV-2 滴度高于临床确诊病例的预期。

废水监测是临床监测的一种补充方法，用于衡量新型冠状病毒 SARS-CoV-2 等新兴传染病的存在和流行。这种创新的数据源可以提高流行病学建模的精度，以了解 SARS-CoV-2 在特定脆弱社区中的外显率。在这里，我们测试了在马萨诸塞州的一个主要城市处理设施收集的废水，并在 2020 年 3 月 18 日至 25 日期间使用 RT检测到显着滴度（每毫升污水 57 至 303 个拷贝）的N基因中的SARS-CoV-2 RNA -qPCR。我们通过 Sanger 对来自S的 PCR 产物进行测序验证了对 SARS-CoV-2 的检测基因。截至 3 月 25 日，观察到的病毒滴度显着高于根据马萨诸塞州临床确诊病例的预期。我们的方法是可扩展的，可能有助于模拟 SARS-CoV-2 大流行和未来的爆发。

更新日期：2020-08-20

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