Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.

中文翻译：

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从赞比亚猪粪便分离的哺乳动物正咽病毒的特征。

已经在人类，牲畜和野生动物中鉴定出哺乳动物正咽病毒（MRV）；这种广泛的寄主范围允许单个MRV传播到多个物种。尽管已经报告了人类，家畜和野生动植物之间MRV的几种种间传播和基因重排事件，但非洲对MRV的遗传多样性和地理分布知之甚少。在这项研究中，我们报告了在赞比亚猪群中流通的MRVs的首次分离和鉴定。在我们的筛选中，通过逆转录PCR在从猪收集的粪便样本中检测到了19.7％（29/147）的MRV基因组。成功分离了三种传染性MRV菌株（MRV-85，MRV-96和MRV-117），并对它们的完整基因组进行了测序。基于分离的MRV的完整基因组序列的重组分析表明，MRV-96与蝙蝠分离的MRV共享S3片段，MRV-117的L1和M3片段分别来自蝙蝠和人类MRV。我们的结果表明，分离的MRVs是通过种间传播的基因重组事件出现的。鉴于缺乏有关非洲MRV的信息，需要进一步监测在人类，家畜和野生动物之间传播的MRV，以评估对人类和动物的潜在风险。我们的结果表明，分离的MRVs是通过种间传播的基因重组事件出现的。鉴于缺乏有关非洲MRV的信息，需要进一步监测在人类，家畜和野生动物之间传播的MRV，以评估对人类和动物的潜在风险。我们的结果表明，分离的MRVs是通过种间传播的基因重组事件出现的。鉴于缺乏有关非洲MRV的信息，需要进一步监测在人类，家畜和野生动物之间传播的MRV，以评估对人类和动物的潜在风险。