当前位置:
X-MOL 学术
›
Methods Appl. Fluoresc.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Evaluating spectral overlap with the degree of quenching in UCP luminescence energy transfer systems.
Methods and Applications in Fluorescence ( IF 3.2 ) Pub Date : 2020-10-05 , DOI: 10.1088/2050-6120/aba87f Letitia Burgess 1, 2 , Hannah Wilson 1, 2, 3 , Alex R Jones 1, 2, 3, 4 , Sam Hay 1, 2 , Louise S Natrajan 1, 3
Methods and Applications in Fluorescence ( IF 3.2 ) Pub Date : 2020-10-05 , DOI: 10.1088/2050-6120/aba87f Letitia Burgess 1, 2 , Hannah Wilson 1, 2, 3 , Alex R Jones 1, 2, 3, 4 , Sam Hay 1, 2 , Louise S Natrajan 1, 3
Affiliation
The use of organic based fluorophores has been firmly established as a key tool in the biological sciences, with many biological-sensing methods taking advantage of Förster Resonance Energy Transfer (FRET) between different fluorescent organic based dyes following one photon excitation. Nevertheless, the employment of UV-visible absorbing dyes as fluorescent tags and markers typically suffer from several drawbacks including relatively high energy of excitation wavelength, photobleaching and competitive autofluorescence, which often limits their effectiveness and longevity both in vitro and in vivo . As an alternative, lanthanide doped upconverting phosphors (UCP) have emerged as a new class of materials for use in optical imaging and RET sensing; they exhibit high photo- and chemical stability and utilise near infrared excitation. Approaches to sensing a given analyte target employing upconverting phosphors can be achieved by engineering the UCP to operate analogous...
中文翻译:
评估光谱重叠与 UCP 发光能量转移系统中的淬灭程度。
有机基荧光团的使用已被牢固地确立为生物科学中的关键工具,许多生物传感方法在一个光子激发后利用不同荧光有机基染料之间的 Förster 共振能量转移 (FRET)。然而,使用紫外可见光吸收染料作为荧光标签和标记物通常存在几个缺点,包括相对较高的激发波长能量、光漂白和竞争性自发荧光,这通常限制了它们在体外和体内的有效性和寿命。作为替代方案,镧系元素掺杂的上转换磷光体 (UCP) 已成为一类用于光学成像和 RET 传感的新型材料。它们表现出很高的光和化学稳定性,并利用近红外激发。
更新日期:2020-10-06
中文翻译:
评估光谱重叠与 UCP 发光能量转移系统中的淬灭程度。
有机基荧光团的使用已被牢固地确立为生物科学中的关键工具,许多生物传感方法在一个光子激发后利用不同荧光有机基染料之间的 Förster 共振能量转移 (FRET)。然而,使用紫外可见光吸收染料作为荧光标签和标记物通常存在几个缺点,包括相对较高的激发波长能量、光漂白和竞争性自发荧光,这通常限制了它们在体外和体内的有效性和寿命。作为替代方案,镧系元素掺杂的上转换磷光体 (UCP) 已成为一类用于光学成像和 RET 传感的新型材料。它们表现出很高的光和化学稳定性,并利用近红外激发。