Biomedical Materials ( IF 4 ) Pub Date : 2020-12-16 , DOI: 10.1088/1748-605x/aba7d4 Rui Xu 1 , Xiaojie Lian 1, 2, 3 , Liqin Zhao 1, 2 , Yan Wei 1, 2 , Dingyu Hou 1 , Baolong Niu 4 , Quanyou Zhang 1, 2 , Di Huang 1, 2 , Fen Li 5 , Sheng Gao 6
Calcium sulfate (CS) bone cements have been used as bone substitutes for a long time, but their clinical use is currently limited due to their rapid degradation rate and brittleness. This work aimed to study the effect of α-tricalcium phosphate (α-TCP) and silk fibroin nanofibers (SFF) on CS bone cements. The bone cements were prepared from α-CS hemihydrate (α-CSH), calcium sulfate dihydrate (CSD; as a setting accelerator) and varying α-TCP contents (0%, 5%, 10%, 15%, 20% and 25%), with SFF solution or deionized water as the solidification solution at the same liquid/solid ratio. Scanning electron microscopy, particle size distribution, x-ray diffraction and Fourier transform infrared spectroscopy were used to measure the composition and characterize the properties of the materials. The compressive strength, setting time and weight loss rate of samples were also tested. Cytotoxicity was evaluated by a Cell Counting Kit-8 assay. The results suggest that the tuning of α-TCP and SFF has an important role in determining the compressive strength and degradation rate of CS bone cements, and the properties could be changed by varying the content of α-TCP. Moreover, cell experiments showed no toxicity of the samples towards MC3T3 cells. Thus, the materials prepared from α-CSH, CSD, α-TCP and SFF in this work could provide the basis for research into CS-based bone repair materials.
中文翻译:
磷酸钙和丝素蛋白纳米纤维调谐对硫酸钙骨水泥性能的影响
硫酸钙(CS)骨水泥作为骨替代物使用已久,但由于其降解速度快且易碎,目前其临床应用受到限制。本工作旨在研究 α-磷酸三钙 (α-TCP) 和丝素蛋白纳米纤维 (SFF) 对 CS 骨水泥的影响。骨水泥由 α-CS 半水合物(α-CSH)、二水硫酸钙(CSD;作为促凝剂)和不同的 α-TCP 含量(0%、5%、10%、15%、20% 和 25 %),以 SFF 溶液或去离子水为固化溶液,液固比相同。扫描电子显微镜、粒度分布、X射线衍射和傅里叶变换红外光谱用于测量材料的成分和表征材料的性能。抗压强度,还测试了样品的凝固时间和失重率。通过 Cell Counting Kit-8 测定评估细胞毒性。结果表明,α-TCP 和 SFF 的调节在决定 CS 骨水泥的抗压强度和降解率方面具有重要作用,并且可以通过改变 α-TCP 的含量来改变其性能。此外,细胞实验表明样品对 MC3T3 细胞没有毒性。因此,本工作以α-CSH、CSD、α-TCP和SFF制备的材料可为CS基骨修复材料的研究提供基础。并且可以通过改变α-TCP的含量来改变性质。此外,细胞实验表明样品对 MC3T3 细胞没有毒性。因此,本工作以α-CSH、CSD、α-TCP和SFF制备的材料可为CS基骨修复材料的研究提供基础。并且可以通过改变α-TCP的含量来改变性质。此外,细胞实验表明样品对 MC3T3 细胞没有毒性。因此,本工作以α-CSH、CSD、α-TCP和SFF制备的材料可为CS基骨修复材料的研究提供基础。