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Antigen Retrieval and Its Effect on the MALDI-MSI of Lipids in Formalin-Fixed Paraffin-Embedded Tissue.
Journal of the American Society for Mass Spectrometry ( IF 3.2 ) Pub Date : 2020-07-22 , DOI: 10.1021/jasms.0c00208 Vanna Denti 1 , Isabella Piga 1 , Sonia Guarnerio 2 , Francesca Clerici 1 , Mariia Ivanova 1 , Clizia Chinello 1 , Giuseppe Paglia 1 , Fulvio Magni 1 , Andrew Smith 1
Journal of the American Society for Mass Spectrometry ( IF 3.2 ) Pub Date : 2020-07-22 , DOI: 10.1021/jasms.0c00208 Vanna Denti 1 , Isabella Piga 1 , Sonia Guarnerio 2 , Francesca Clerici 1 , Mariia Ivanova 1 , Clizia Chinello 1 , Giuseppe Paglia 1 , Fulvio Magni 1 , Andrew Smith 1
Affiliation
Formalin-fixed paraffin-embedded (FFPE) tissue represents the primary source of clinical tissue and is routinely used in MALDI-MSI studies. However, it is not particularly suitable for lipidomics imaging given that many species are depleted during tissue processing. Irrespective, a number of solvent-resistant lipids remain, but their extraction may be hindered by the cross-link between proteins. Therefore, an antigen retrieval step could enable the extraction of a greater number of lipids and may provide information that is complementary to that which can be obtained from other biomolecules, such as proteins. In this short communication, we aim to address the effect of performing antigen retrieval prior to MALDI-MSI of lipids in FFPE tissue. As a result, an increased number of lipid signals could be detected and may have derived from lipid species that are known to be implicated in the lipid-protein cross-linking that is formed as a result of formalin fixation. Human renal cancer tissue was used as a proof of concept to determine whether using these detected lipid signals were also able to highlight the histopathological regions that were present. These preliminary findings may highlight the potential to enhance the clinical relevance of the lipidomic information obtained from FFPE tissue.
中文翻译:
抗原修复及其对福尔马林固定石蜡包埋组织中脂质 MALDI-MSI 的影响。
福尔马林固定石蜡包埋 (FFPE) 组织是临床组织的主要来源,常用于 MALDI-MSI 研究。然而,鉴于许多物种在组织处理过程中被耗尽,它并不特别适合脂质组学成像。无论如何,仍然存在许多耐溶剂脂质,但蛋白质之间的交联可能会阻碍它们的提取。因此,抗原修复步骤可以提取更多的脂质,并可以提供与从其他生物分子(如蛋白质)获得的信息互补的信息。在这篇简短的交流中,我们旨在解决在 FFPE 组织中脂质的 MALDI-MSI 之前进行抗原修复的影响。其结果,可以检测到数量增加的脂质信号,这些信号可能来自已知与福尔马林固定形成的脂质-蛋白质交联有关的脂质种类。人类肾癌组织被用作概念证明,以确定使用这些检测到的脂质信号是否也能够突出存在的组织病理学区域。这些初步发现可能突出了增强从 FFPE 组织获得的脂质组学信息的临床相关性的潜力。
更新日期:2020-07-22
中文翻译:
抗原修复及其对福尔马林固定石蜡包埋组织中脂质 MALDI-MSI 的影响。
福尔马林固定石蜡包埋 (FFPE) 组织是临床组织的主要来源,常用于 MALDI-MSI 研究。然而,鉴于许多物种在组织处理过程中被耗尽,它并不特别适合脂质组学成像。无论如何,仍然存在许多耐溶剂脂质,但蛋白质之间的交联可能会阻碍它们的提取。因此,抗原修复步骤可以提取更多的脂质,并可以提供与从其他生物分子(如蛋白质)获得的信息互补的信息。在这篇简短的交流中,我们旨在解决在 FFPE 组织中脂质的 MALDI-MSI 之前进行抗原修复的影响。其结果,可以检测到数量增加的脂质信号,这些信号可能来自已知与福尔马林固定形成的脂质-蛋白质交联有关的脂质种类。人类肾癌组织被用作概念证明,以确定使用这些检测到的脂质信号是否也能够突出存在的组织病理学区域。这些初步发现可能突出了增强从 FFPE 组织获得的脂质组学信息的临床相关性的潜力。