Silver nanoparticles (Ag NPs) are invested in various sectors and are becoming more persistent in our ambient environment with potential risk on our health and the ecosystems. The current study aims to investigate the histological, histochemical and ultrastructural hepatic changes that might be induced by 10 nm silver nanomaterials. Male mice (BALB/C) were exposed for 35 injections of daily dose of 10 nm Ag NPs (2 mg/kg). Liver tissues were subjected to examination by light and electron microscopy for histological, histochemical and ultrastructural alterations. Exposure to Ag NPs induced Kupffer cells hyperplasia, sinusoidal dilatation, apoptosis, ground glass hepatocytes appearance, nuclear changes, inflammatory cells infiltration, hepatocytes degeneration and necrosis. In addition, 10 nm Ag NPs induced histochemical alterations mainly glycogen depletion with no hemosiderin precipitation. Moreover, these nanomaterials exhibited ultrastructure alterations including mitochondrial swelling and cristolysis, cytoplasmic vacuolation, apoptosis, multilammellar myelin figures formation and endoplasmic destruction and reduction. The findings revealed that Ag NPs can induce alterations in the hepatic tissues, the chemical components of the hepatocytes and in the ultrastructure of the liver. One may also conclude that small size Ag NPs, which are increasingly used in human products could cause various toxigenic responses to all hepatic tissue components.

中文翻译：

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10 nm 银纳米粒子诱导的肝组织病理学和超微结构改变。

银纳米粒子 (Ag NPs) 投资于各个领域，并且在我们的周围环境中变得更加持久，对我们的健康和生态系统具有潜在风险。目前的研究旨在调查可能由 10 nm 银纳米材料引起的组织学、组织化学和超微结构肝脏变化。雄性小鼠 (BALB/C) 每天注射 35 次 10 nm Ag NPs (2 mg/kg)。通过光学和电子显微镜检查肝组织的组织学、组织化学和超微结构改变。暴露于 Ag NPs 可诱导 Kupffer 细胞增生、肝窦扩张、凋亡、毛玻璃样肝细胞出现、细胞核改变、炎性细胞浸润、肝细胞变性和坏死。此外，10 nm Ag NPs 诱导的组织化学变化主要是糖原消耗，没有含铁血黄素沉淀。此外，这些纳米材料表现出超微结构改变，包括线粒体肿胀和晶状体溶解、细胞质空泡化、细胞凋亡、多层髓鞘形成和内质破坏和减少。研究结果表明，Ag NPs 可以诱导肝组织、肝细胞化学成分和肝脏超微结构的改变。人们还可以得出结论，越来越多地用于人类产品中的小尺寸 Ag NPs 可能会对所有肝组织成分产生各种产毒反应。细胞凋亡、多层髓鞘形成和内质破坏和减少。研究结果表明，Ag NPs 可以诱导肝组织、肝细胞化学成分和肝脏超微结构的改变。人们还可以得出结论，越来越多地用于人类产品中的小尺寸 Ag NPs 可能会对所有肝组织成分产生各种产毒反应。细胞凋亡、多层髓鞘形成和内质破坏和减少。研究结果表明，Ag NPs 可以诱导肝组织、肝细胞化学成分和肝脏超微结构的改变。人们还可以得出结论，越来越多地用于人类产品中的小尺寸 Ag NPs 可能会对所有肝组织成分产生各种产毒反应。