To compare the effects of different carbon sources on physiological aspects, especially medicinal alkaloid biosynthesis and related gene expression in Catharantus roseus (L.) G.Don, we employed sucrose and sorbitol with two concentrations (87.64 mM, the equimolar concentration of sucrose in MS basal medium, and 150 mM) on the plant’s shoots in vitro in presence of 100 μM methyl jasmonate. The production of plant alkaloids including vincristine, vinblastine, ajmalicine, vindoline and catharantine and their biosynthetic and regulatory gene expression was measured. Both treatments had incremental effects on alkaloid production, upregulated the mitogen-activated protein kinase3 (MAPK3) and a downstream responsive transcription factor, ORCA3, which resulted in elevated transcript contents of the important genes in terpenoid indol alkaloids biosynthetic pathway including peroxidase1 (PRX1), geissoschizine synthase (GS), strictosidine synthase (STR) and deacetylvindoline acetyltransferase (DAT). Defensive responses such as antioxidant enzymes (catalase, peroxidase and superoxide dismutase) activities and non-enzymatic metabolites (total phenolics, flavonoids and carotenoids) contents increased under both treatments but the effects of sorbitol were stronger. Reduced fresh weight and chlorophylls contents, increased malondialdehyde (MDA) and carotenoid contents were shown after a week under all employed treatments. It seems that replacement of sucrose with sorbitol and also, increased concentrations of both carbon sources via increasing osmotic pressure make stressful conditions for the plant especially in longer times.

为了比较不同碳源对生理方面的影响，特别是药用生物碱的生物合成和相关基因在长春花（L.）G.Don中的表达，我们使用了蔗糖和山梨糖醇两种浓度（87.64 mM，等摩尔浓度的MS中的蔗糖）基础培养基和150 mM的植物芽）在100μM茉莉酸甲酯存在下 测量了包括长春新碱，长春碱，苦艾碱，长春碱和长春花碱的植物生物碱的产生及其生物合成和调控基因的表达。两种处理都对生物碱产生有增量影响，上调了丝裂原激活的蛋白激酶3（MAPK3）和下游响应转录因子ORCA3，导致萜烯类吲哚生物碱生物合成途径中重要基因的转录物含量升高，包括过氧化物酶1（PRX1）， Geissoschizine合酶（GS），strictosidine合酶（STR）和去乙酰基vindoline乙酰转移酶（DAT）。防御反应，例如抗氧化酶（过氧化氢酶，过氧化物酶和超氧化物歧化酶）活性和非酶代谢物（总酚，两种处理均增加了类黄酮和类胡萝卜素的含量，但山梨醇的作用更强。在所有采用的处理方法下，一周后均显示出鲜重和叶绿素含量降低，丙二醛（MDA）和类胡萝卜素含量增加。似乎用山梨糖醇代替蔗糖，以及通过增加渗透压增加两种碳源的浓度，使植物处于压力状态，尤其是在更长的时间内。