当前位置: X-MOL 学术J. Biochem. Mol. Toxicol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Local anesthetic articaine ameliorates LPS-induced acute kidney injury via inhibition of NF-ĸB activation and the NLRP3 inflammasome pathway.
Journal of Biochemical and Molecular Toxicology ( IF 3.6 ) Pub Date : 2020-07-20 , DOI: 10.1002/jbt.22554
Guanjie Zhao 1 , Shan Lu 2 , Linlin Li 2 , Xiaodi Fan 2
Affiliation  

The present study was conducted to determine the protective effect of articaine (ART) in an lipopolysaccharide (LPS)‐induced acute kidney injury (AKI) animal model. The results suggest ART causes a significant decrease in serum blood urea nitrogen, creatinine, and serum cystatin C level, showing a protective effect against LPS‐induced AKI. This has been further supported by histopathological findings of kidney tissues. The level of tumor necrosis factor‐α, interleukin (IL)‐6, and IL‐1β in serum and kidney tissues was remarkably inhibited by ART in a dose‐dependent manner. ART causes a significant reduction of malondialdehyde and increases the activities of glutathione and superoxide dismutase with an increase in dose as compared to the LPS‐treated group. Moreover, the ART‐treated group showed dose‐dependent inhibition of LPS‐induced nuclear factor‐κB activation and TLR4 expression as confirmed by Western blot analysis. The level of Bcl‐2 family genes (Bcl‐2 and Bax) was restored near to normal by ART. Collectively, all the above results indicated that ART had protective effects against LPS‐induced AKI by blocking inflammatory and oxidative responses.

中文翻译:

局麻药可通过抑制NF-ĸB激活和NLRP3炎性体途径改善LPS诱导的急性肾脏损伤。

进行本研究是为了确定青蒿素(ART)在脂多糖(LPS)诱导的急性肾损伤(AKI)动物模型中的保护作用。结果表明,抗逆转录病毒治疗可显着降低血清尿素氮,肌酐和血清胱抑素C水平,显示出对LPS诱导的AKI的保护作用。肾脏组织的组织病理学发现进一步支持了这一点。血清和肾脏组织中的肿瘤坏死因子α,白介素(IL)-6和IL-1β的水平受到剂量依赖性ART的显着抑制。与LPS治疗组相比,ART可使丙二醛显着减少,并增加了谷胱甘肽和超氧化物歧化酶的活性。此外,Western印迹分析证实,ART治疗组对LPS诱导的核因子κB活化和TLR4表达具有剂量依赖性抑制作用。Bcl-2家族基因(Bcl-2和Bax)的水平已通过ART恢复至接近正常水平。总的来说,以上所有结果表明,ART通过阻断炎症和氧化反应,对LPS诱导的AKI具有保护作用。
更新日期:2020-07-20
down
wechat
bug