1 BACKGROUND

Incompatible ABO blood transfusion may cause a severe intravascular hemolytic reaction that can be life threatening. In the case of transfusion of incompatible blood and the presence of a double population of red blood cells, establishing blood type is challenging. In addition to trauma cases and conflict regions, polytransfused patients may need to have their true phenotype resolved as part of the evaluation of alloimmunization.

During the past few years several trauma cases were admitted to our medical center from the Syrian civil war. Some of these cases may have been transfused with incompatible blood in Syria prior to their transfer to Israel (table describing cases available upon request). The double population of red blood cells observed in their blood samples upon arrival to our blood bank caused difficulties in determining accurate blood type of the patients by serology.

One of these cases was described recently when blood type was determined mistakenly by genotyping following an incompatible blood transfusion, resulting in further incompatible blood product transfusion (Akria et al., 2017). Thus, our laboratory sought a solution of blood typing using flow cytometry to determine the true recipient blood type rapidly and simply.

中文翻译：

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通过未成熟的网织红细胞免疫表型流式细胞术检测血型。

1 背景

不相容的 ABO 输血可能导致严重的血管内溶血反应，可能危及生命。在输血不相容的血液和双胞胎红细胞的情况下，确定血型具有挑战性。除了创伤病例和冲突地区，多输血患者可能需要解决他们的真实表型，作为同种免疫评估的一部分。

在过去的几年里，我们的医疗中心因叙利亚内战而收治了几起创伤病例。其中一些病例在转移到以色列之前可能在叙利亚输过不相容的血液（可根据要求提供病例描述表）。到达我们血库时在血液样本中观察到的双倍红细胞群导致难以通过血清学确定患者的准确血型。

最近描述了其中一个案例，在不相容的输血后通过基因分型错误地确定了血型，导致进一步不相容的血液制品输血（Akria et al., 2017）。因此，我们的实验室寻求一种使用流式细胞术进行血型分型的解决方案，以快速、简单地确定真正的受体血型。