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Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during In Vitro Fertilization Surgery.
mSystems ( IF 6.4 ) Pub Date : 2020-07-14 , DOI: 10.1128/msystems.00450-20
Changying Zhao 1, 2, 3 , Zefeng Wei 4 , Junjie Yang 5 , Jiaming Zhang 6 , Chunna Yu 4 , Aijun Yang 4 , Min Zhang 4 , Lin Zhang 4 , Ye Wang 7 , Xiaofeng Mu 7 , Xueyuan Heng 8 , Huijun Yang 9 , Zhongtao Gai 1 , Xuenan Wang 10 , Lei Zhang 2, 3, 11
Affiliation  

Perturbation of vaginal microbiome of reproductive-age women influences all the phases of a woman's reproductive life. Although studies have shown that dynamic changes in vaginal microbiome can affect pregnancy, its role in secondary infertility (i.e., inability to become pregnant or to carry a pregnancy successfully after previous success in delivering a child) and in vitro fertilization (IVF) remains to be unraveled. To determine the vaginal microbiome in women undergoing in vitro fertilization and embryo transfer (IVF-ET) and investigate its potential correlations with hormone stimulation, we recruited 30 patients with secondary infertility and receiving IVF and 92 matched healthy women and analyzed their vaginal microbiome composition using 16S rRNA gene sequencing. Our results show that women suffering from infertility (infertile women) exhibit a significant decrease in microbiome diversity and richness compared with healthy women during the nonovulation period (follicular phase) (P < 0.01), whereas vaginal microbiome of healthy women reveals dramatic fluctuations during ovulation (P < 0.05). Interestingly, infertility patients show no change of the vaginal microbiome under conditions of gonadotropin-releasing hormone (GnRH) agonist and recombinant human chorionic gonadotropin (r-hCG) induction (P > 0.05). Moreover, our results indicate that infertile women show characteristic variations in vaginal microbiome, such as increased abundance of Atopobium, Aerococcus, and Bifidobacterium and decreased abundance of Lactobacillus and Leuconostoc.

中文翻译:

不育妇女阴道微生物组的特征及其在体外受精手术中与激素刺激的潜在相关性。

生殖年龄女性阴道微生物组的扰动影响着女性生殖生活的所有阶段。虽然有研究表明,在阴道微生物动态变化可影响受孕,其在继发性不孕的作用(即不能怀孕或在提供孩子以前的成功后,成功地怀孕)和ñ v ITRO ˚F ertilization(IVF)仍有待揭露。为了确定在女性阴道微生物经历ñ v ITRO ˚F ertilization和Ë mbryo ŧ(IVF-ET),并研究其与激素刺激的潜在相关性,我们招募了30例继发性不育并接受IVF的患者和92名匹配的健康女性,并使用16S rRNA基因测序分析了她们的阴道微生物组组成。我们的研究结果表明,在非排卵期(卵泡期),与不育女性(不育女性)相比,健康女性的微生物组多样性和丰富度显着降低(P < 0.01),而健康女性的阴道微生物组显示排卵期间的剧烈波动(P < 0.05)。有趣的是,不孕症患者表现出的条件下没有阴道微生物的变化ö Ñ adotropin- řeleasing ħ ormone(促性腺激素释放激素)激动剂和- [R ecombinant ħ UMAN Ç horioniconadotropin(R-HCG)诱导(P> 0.05)。此外,我们的结果表明,不育女性在阴道微生物组中表现出特征性变异,例如增加的拟南芥,气球菌双歧杆菌的丰度,以及乳酸杆菌亮粘菌的丰度降低。
更新日期:2020-08-20
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