To evaluate the catalytic esterification performance of proteases in micro-aqueous systems and to study the suitable conditions for maintaining protease activity. It was found that the protease showed better enzyme catalytic activity in the micro-aqueous phase containing 4% boric acid-borax buffer than that of the pure organic phase. The protease activity was easily activated by 0.20 M boric acid-borax buffer, and the enzyme activity was still high for a long time in alkaline environment (pH 8.40–9.60) and under the temperature of 40–55 °C. Experiments using protease and Candida lipase to synthesize sucrose-6-ethyl ester showed that protease had better esterification activity than Candida lipase in the micro-aqueous phase.

中文翻译：

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蛋白酶在微水体系中的催化酯化性能

评价蛋白酶在微水体系中的催化酯化性能，研究维持蛋白酶活性的适宜条件。发现蛋白酶在含4%硼酸-硼砂缓冲液的微水相中比纯有机相表现出更好的酶催化活性。蛋白酶活性很容易被0.20 M的硼酸-硼砂缓冲液激活，在碱性环境（pH 8.40-9.60）和40-55°C的温度下，酶活性仍然很高。用蛋白酶和念珠菌脂肪酶合成蔗糖6-乙酯的实验表明，蛋白酶在微水相中比念珠菌脂肪酶具有更好的酯化活性。