A central question in eukaryotic cell biology asks, during cell division, how is the growth and distribution of organelles regulated to ensure each daughter cell receives an appropriate amount. For vacuoles in budding yeast, there are well described organelle-to-cell size scaling trends as well as inheritance mechanisms involving highly coordinated movements. It is unclear whether such mechanisms are necessary in the symmetrically dividing fission yeast, Schizosaccharomyces pombe , in which random partitioning may be utilized to distribute vacuoles to daughter cells. To address the increasing need for high-throughput analysis, we are augmenting existing semi-automated image processing by developing fully automated machine learning methods for locating vacuoles and segmenting fission yeast cells from brightfield and fluorescence micrographs. All strains studied show qualitative correlations in vacuole-to-cell size scaling trends, i.e. vacuole volume, surface area, and numbe...

中文翻译：

---

粟酒裂殖酵母中空泡的非随机分布。

真核细胞生物学的一个核心问题是，在细胞分裂过程中，细胞器的生长和分布是如何调节的，以确保每个子细胞都能接收到适量的细胞器。对于出芽酵母中的液泡，有很好描述的细胞器到细胞大小缩放趋势以及涉及高度协调运动的遗传机制。目前尚不清楚这种机制在对称分裂的裂殖酵母 Schizosaccharomyces pombe 中是否必要，其中可以利用随机分配将液泡分配给子细胞。为了满足对高通量分析日益增长的需求，我们正在通过开发全自动机器学习方法来增强现有的半自动图像处理，以从明场和荧光显微照片中定位液泡和分割裂殖酵母细胞。