The whole genome sequence of Lactobacillus paracasei DTA72, isolated from healthy infant feces, is reported, along with the Carbohydrates-Active enZymes (CAZymes) analysis and an in silico safety assessment. Strain DTA72 had previously demonstrated some interesting potential probiotic features, such as a good resistance to gastrointestinal conditions and an anti-Listeria activity. The 3.1 Mb sequenced genome consists of 3116 protein-coding sequences distributed on 340 SEED subsystems. In the present study, we analyzed the fermentation capability of strain DTA72 on six different carbohydrate sources, namely, glucose, fructose, lactose, galactose, xylose, and inulin by using phenotypical and genomic approaches. Interestingly, L. paracasei DTA72 evidenced the best growth performances on inulin with a much shorter lag phase and higher number of cells at the stationary phase in comparison with all the sugars tested. The CAZyme analysis using the predicted amino acid sequences detected 80 enzymes, distributed into the five CAZymes classes. Moreover, the in silico analysis revealed the absence of blood hemolytic genes, transmissible antibiotic resistances, and plasmids in DTA72. The results described in this study, together with those previously reported and particularly the strong capability to utilize inulin as energy source, make DTA72 a very interesting potential probiotic strain to be considered for the production of synbiotic foods. The complete genome data have been deposited in GenBank under the accession number WUJH00000000.

中文翻译：

---

副干酪乳杆菌 DTA72 的完整基因组序列和碳水化合物活性酶 (CAZymes) 分析，这是一种具有强大菊粉使用能力的潜在益生菌菌株

报告了从健康婴儿粪便中分离的副干酪乳杆菌 DTA72 的全基因组序列，以及碳水化合物活性酶 (CAZymes) 分析和计算机安全性评估。菌株 DTA72 之前已经证明了一些有趣的潜在益生菌特性，例如对胃肠道疾病的良好抵抗力和抗李斯特菌活性。3.1 Mb 测序的基因组由分布在 340 个 SEED 子系统上的 3116 个蛋白质编码序列组成。在本研究中，我们通过表型和基因组方法分析了菌株 DTA72 对六种不同碳水化合物来源的发酵能力，即葡萄糖、果糖、乳糖、半乳糖、木糖和菊粉。有趣的是，L。与所有测试的糖相比，副干酪 DTA72 证明了菊粉的最佳生长性能，具有更短的滞后期和更多的静止期细胞数。使用预测的氨基酸序列的 CAZyme 分析检测到 80 种酶，分布在五个 CAZymes 类别中。此外，计算机分析显示 DTA72 中不存在血液溶血基因、可传播的抗生素抗性和质粒。本研究中描述的结果，加上之前报道的结果，特别是利用菊粉作为能源的强大能力，使 DTA72 成为一种非常有趣的潜在益生菌菌株，可考虑用于生产合生元食品。完整的基因组数据已存入GenBank，登录号为WUJH00000000。