Circular RNAs (circRNAs) are a novel class of noncoding RNAs that are widely expressed in human disease. However, circRNAs expression profile and potential mechanism in osteoporosis pathogenesis remain to be further studied. In the present study, a total of 69 circRNAs were identified to be abnormally expressed in osteoporosis patient samples by microarray and bioinformatics analyses. We found that circ_0011269 was notably downregulated in osteoporosis (fold change, 3.94). By means of miRanda algorithm, we constructed the interaction network of circ_0011269‐miRNAs in osteoporosis based on target binding and miR‐122 was enrolled in the network. Dual‐luciferase reporter assay verified the target relationship of miR‐122 and circ_0011269/RUNX2. The expression of circ_0011269 and RUNX2 were gradually increased during osteogenic differentiation while miR‐122 exhibited a decreased expression. Moreover, overexpression of circ_0011269 could promote RUNX2 expression and inhibit osteoporosis. In summary, this study found that circ_0011269 sponges miR‐122 to regulate RUNX2 expression and promotes osteoporosis progression.

中文翻译：

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环状 RNA circ_0011269 海绵 miR-122 调节 RUNX2 表达并促进骨质疏松症进展。

环状RNA（circRNA）是一类新型非编码RNA，在人类疾病中广泛表达。然而，circRNA的表达谱及其在骨质疏松发病机制中的潜在机制仍有待进一步研究。在本研究中，通过微阵列和生物信息学分析，总共鉴定出 69 个 circRNA 在骨质疏松症患者样本中异常表达。我们发现 circ_0011269 在骨质疏松症中显着下调（倍数变化，3.94）。通过miRanda算法，我们构建了基于靶标结合的circ_0011269-miRNA在骨质疏松症中的相互作用网络，并且miR-122被纳入该网络。双荧光素酶报告基因测定验证了 miR-122 和 circ_0011269/RUNX2 的靶标关系。circ_0011269和RUNX2的表达在成骨分化过程中逐渐增加，而miR-122的表达减少。此外，circ_0011269的过度表达可以促进RUNX2的表达并抑制骨质疏松。总之，本研究发现 circ_0011269 海绵 miR-122 调节 RUNX2 表达并促进骨质疏松症进展。