Regional distribution of adipose tissue is an important factor in conferring cardiometabolic risk and obesity-related morbidity. We tested thehypothesis that human visceral adipose tissue (VAT) impairs glucose homeostasis, whereas subcutaneous glutealfemoral adipose tissue (GFAT)protects against the development of impaired glucose homeostasis in mice.VAT and GFAT were collected from patients undergoing bariatric surgery and grafted onto the epididymal adipose tissue of weight and age-matched severe combined immunodeficient mice. SHAM mice underwent surgery without transplant of tissue. Mice were fed a high-fat diet after xenograft. Energy homeostasis, glucose metabolism and insulin sensitivity were assessed six weeks later.Xenograftof human adipose tissues was successful as determined by histology, immunohistochemical evaluation of collagen deposition and angiogenesis, and maintenance of lipolytic function. Adipose tissue transplant did not affect energy expenditure, food intake, whole-body substrate partitioning, or plasma free fatty acid, triglyceride and insulin levels. Fasting blood glucose was significantly reduced in GFAT and VAT compared with SHAM, whereas glucose tolerance was only improved in mice transplanted with VAT compared with SHAM mice. This improvement was not associated with differences in whole-body insulin sensitivity or plasma insulin between groups. Together, these data suggest thatVAT improves glycemic control and GFAT does not protect against the development of high fat diet-induced glucose intolerance. Hence, the intrinsic properties of VAT and GFAT do not necessarily explain the postulated negative and positive effects of these adipose tissue depots on metabolic health.

中文翻译：

---

在饮食诱发的肥胖小鼠模型中，人内脏和臀股脂肪组织移植对血糖控制的影响。

脂肪组织的区域分布是赋予心脏代谢风险和与肥胖相关的发病率的重要因素。我们测试了以下假设：人内脏脂肪组织（VAT）损害了葡萄糖稳态，而皮下臀股脂肪组织（GFAT）防止了小鼠体内葡萄糖稳态的发展。体重和年龄相匹配的严重联合免疫缺陷小鼠的组织。SHAM小鼠接受了手术，没有组织移植。异种移植后，给小鼠喂食高脂饮食。六周后评估能量稳态，葡萄糖代谢和胰岛素敏感性。通过组织学确定异种移植人类脂肪组织成功，胶原组织沉积和血管生成的免疫组织化学评估以及脂解功能的维持。脂肪组织移植不影响能量消耗，食物摄入，全身底物分配或血浆游离脂肪酸，甘油三酸酯和胰岛素水平。与SHAM相比，GFAT和VAT中的空腹血糖明显降低，而与SHAM小鼠相比，只有VAT移植的小鼠才提高了葡萄糖耐量。这种改善与两组之间的全身胰岛素敏感性或血浆胰岛素的差异无关。总之，这些数据表明增值税可改善血糖控制，而GFAT不能防止高脂饮食引起的葡萄糖耐量异常。因此，