当前位置: X-MOL 学术Lipids › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Measurement of Enzymatic and Nonenzymatic Polyunsaturated Fatty Acid Oxidation Products in Plasma and Urine of Macular Degeneration Using LC-QTOF-MS/MS.
Lipids ( IF 1.9 ) Pub Date : 2020-06-30 , DOI: 10.1002/lipd.12264
Ho Hang Leung 1 , Kin Sum Leung 1 , Thierry Durand 2 , Jean-Marie Galano 2 , Jetty Chung-Yung Lee 1
Affiliation  

Oxidized polyunsaturated fatty acids (PUFA) are associated to pathogenesis of diseases including cardiovascular and neurodegeneration. The novel products are not only biomarkers but also lipid mediators in gene regulation and signaling pathways. Herein, simultaneous quantitation of 28 products derived from nonenzymatic and enzymatic oxidation of PUFA i.e. 5‐, 15‐F2t‐isoprostanes, 7‐, 17‐F2t‐dihomo‐isoprostanes, 7‐, 17‐F2t‐dihomo‐isofurans, 5‐, 8‐, 18‐F3t‐isoprostanes, 4‐, 10‐, 13‐, 14‐, 20‐F4t‐neuroprostanes, 5‐, 8‐, 9‐, 11‐,12‐, 15‐, 20‐HETE, 4‐, 7‐, 11‐, 14‐, 17‐HDHA, RvE1, and NPD1 using LC‐(ESI)‐QTOF‐MS/MS was developed. These products were measurable in a single sample and the analytical time was relative short (~15 min). Furthermore, we showed that the use of internal standards is a requisite to normalize matrix effects and preparation loss for the quantitation. Validation assays indicated the method to be robust for plasma and mid‐stream urine sample analysis in particular from those of age‐related macular degeneration subjects, where the accuracy of quantitation displayed good repeatability.

中文翻译:

使用 LC-QTOF-MS/MS 测量黄斑变性血浆和尿液中的酶促和非酶促多不饱和脂肪酸氧化产物。

氧化多不饱和脂肪酸 (PUFA) 与包括心血管和神经变性在内的疾病的发病机制有关。新产品不仅是生物标志物,也是基因调控和信号通路中的脂质介质。在此,同时定量 28 种衍生自 PUFA 的非酶促和酶促氧化的产物,5-, 15-F 2t -isoprostanes, 7-, 17-F 2t -dihomo-isoprostanes, 7-, 17-F 2t -dihomo-isofurans, 5-, 8-, 18-F 3t -isoprostanes, 4-, 10-, 13-, 14-, 20-F 4t-神经前列腺素,5-, 8-, 9-, 11-,12-, 15-, 20-HETE, 4-, 7-, 11-, 14-, 17-HDHA, RvE1, and NPD1 using LC-(ESI )-QTOF-MS/MS 的开发。这些产品可在单个样品中进行测量,分析时间相对较短(约 15 分钟)。此外,我们表明使用内标是标准化基质效应和定量准备损失的必要条件。验证分析表明该方法对于血浆和中段尿样分析是可靠的,特别是来自年龄相关性黄斑变性受试者的血浆和中段尿样分析,其中定量的准确性显示出良好的可重复性。
更新日期:2020-06-30
down
wechat
bug