Objective: An insufficient mineralization (hypomineralization) in the teeth during the maturation stage of amelogenesis cause defects in 3-44% of children. Here, we describe for the first time the microbiota associated with these defects and compared it to healthy teeth within the same subjects. Methods: Supragingival dental plaque was sampled from healthy and affected teeth from 25 children with molar-incisor hypomineralization (MIH). Total DNA was extracted and the 16S rRNA gene was sequenced by Illumina sequencing in order to describe the bacterial composition. Results: We detected a higher bacterial diversity in MIH samples, suggesting better bacterial adhesion or higher number of niches in those surfaces. We found the genera Catonella, Fusobacterium, Campylobacter, Tannerella, Centipeda, Streptobacillus, Alloprevotella and Selenomonas associated with hypomineralized teeth, whereas Rothia and Lautropia were associated with healthy sites. Conclusion: The higher protein content of MIH-affected teeth could favour colonization by proteolytic microorganisms. The over-representation of bacteria associated with endodontic infections and periodontal pathologies suggests that, in addition to promote caries development, MIH could increase the risk of other oral diseases.

中文翻译：

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磨牙－切牙矿化不足病灶的微生物学。初步研究。

目的：牙釉质成熟阶段牙齿中的矿物质不足（次矿物质化）不足，会导致3-44％的儿童出现缺陷。在这里，我们首次描述了与这些缺陷相关的微生物群，并将其与相同受试者中的健康牙齿进行了比较。方法：从25例磨牙-门牙矿化度低（MIH）儿童的健康牙齿和受影响的牙齿中取样龈上牙菌斑。提取总DNA，并通过Illumina测序对16S rRNA基因进行测序，以描述细菌组成。结果：我们在MIH样品中检测到较高的细菌多样性，表明这些表面的细菌附着力更好或壁ni数量更高。我们发现Catonella，Fusobacter，Campylobacter，Tannerella，Centipeda，链霉菌属，异位藻和硒单胞菌与牙齿矿化不足有关，而Rothia和Lautropia与健康部位有关。结论：受MIH影响的牙齿中较高的蛋白质含量有助于蛋白水解微生物定植。与牙髓感染和牙周病相关的细菌的过度表达表明，MIH除了促进龋齿的发展外，还可能增加其他口腔疾病的风险。