Introduction. Burkholderia cepacia complex (Bcc) bacteria, currently consisting of 23 closely related species, and Burkholderia gladioli , can cause serious and difficult-to-treat infections in people with cystic fibrosis. Identifying Burkholderia bacteria to the species level is considered important for understanding epidemiology and infection control, and predicting clinical outcomes. Matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF) is a rapid method recently introduced in clinical laboratories for bacterial species-level identification. However, reports on the ability of MALDI-TOF to accurately identify Bcc to the species level are mixed. Aim. The aim of this project was to evaluate the accuracy of MALDI-TOF using the Biotyper and VITEK MS systems in identifying isolates from 22 different Bcc species and B. gladioli compared to recA gene sequencing, which is considered the current gold standard for Bcc. Methodology. To capture maximum intra-species variation, phylogenetic trees were constructed from concatenated multi-locus sequence typing alleles and clustered with a novel k-medoids approach. One hundred isolates representing 22 Bcc species, plus B. gladioli , were assessed for bacterial identifications using the two MALDI-TOF systems. Results. At the genus level, 100 and 97.0 % of isolates were confidently identified as Burkholderia by the Biotyper and VITEK MS systems, respectively; moreover, 26.0 and 67.0 % of the isolates were correctly identified to the species level, respectively. In many, but not all, cases of species misidentification or failed identification, a representative library for that species was lacking. Conclusion. Currently available MALDI-TOF systems frequently do not accurately identify Bcc bacteria to the species level.

中文翻译：

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基质辅助激光解吸/电离飞行时间质谱仪可在临床微生物学实验室中准确鉴定洋葱伯克霍尔德菌和伯克霍尔德菌。

介绍。 目前由23个密切相关的物种组成的伯克霍尔德菌洋葱伯克霍尔德菌（Bcc）细菌和剑兰伯克霍尔德菌可对囊性纤维化患者造成严重且难以治疗的感染。将伯克霍尔德氏菌鉴定到物种水平被认为对于理解流行病学和感染控制以及预测临床结果非常重要。基质辅助激光解吸/电离飞行时间质谱仪（MALDI-TOF）是临床实验室中最近引入的一种快速方法，用于细菌种类的鉴定。但是，关于MALDI-TOF准确识别物种中Bcc的能力的报道不一。目标。 该项目的目的是评估与recA基因测序相比，使用Biotyper和VITEK MS系统鉴定MALDI-TOF的准确性，以鉴定来自22种不同Bcc物种和剑兰的分离物，recA基因测序被认为是当前Bcc的黄金标准。方法。为了捕获最大的种内变异，从串联的多位点序列分型等位基因构建系统进化树，并用新颖的k-medoids方法聚类。使用两个MALDI-TOF系统评估了代表22个Bcc物种的100个分离株以及剑兰芽孢杆菌的细菌鉴定。结果。在属水平上，有100％和97.0％的分离株被确定为 Biotyper和VITEK MS系统分别提供伯克霍尔德氏菌；此外，分别在物种水平上正确鉴定出了26.0％和67.0％的分离株。在许多（但不是全部）物种错误识别或识别失败的案例中，缺少该物种的代表性库。结论。当前可用的MALDI-TOF系统经常无法准确地识别Bcc细菌到物种水平。