This study investigated the effect of sublethal concentrations (0.0, 0.3, 0.6, and 1.2 mg·L-1) of herbicide oxadiazon (ODZ) on the activities of Ca2+-ATPase and Na+/K+-ATPase in juvenile Clarias gariepinus. One hundred eighty juveniles of Clarias gariepinus (mean weight 58.88 ± 1.24 g and mean length 22.34 ± 1.52 cm) were randomly divided into four groups and exposed to sublethal concentrations (0.00, 0.3, 0.6, and 1.2 mg·L-1 ODZ) for 21 days in a static renewal bioassay system in which the herbicide and water were replaced completely every day. The changes in Ca2+- and Na+/K+-ATPase activities in the gill, kidney, liver, and heart of the fish were assayed on days 1, 7, 14, and 21. The result showed significant alteration in the activity of these membrane-bound enzymes. There was duration and concentration-dependent significant (p < 0.05) increase in Ca2+-ATPase activity in the treatment groups when compared with the control. The Na+/K+-ATPase activity was significantly (p < 0.05) inhibited in all the tissues when compared with control. The observed alterations in the activities of both Ca2+-ATPase and Na+/K+-ATPase in this study may be indication of impaired ionic transport and imbalance in the fish which may trigger other biochemical, physiological, and even neurological consequences that may compromise several body functions. The alteration of the ATPase activities in C. gariepinus by ODZ is likely to affect the ATP usage and energy metabolism in the fish serious repercussions on Ca2+ homeostasis, Na+/K+ sodium pump, and Ca2+/Na+ exchanger The results suggested that assay of the enzymes could be used as a biomarker of water pollution.

中文翻译：

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暴露于恶草酮的淡水非洲鲶鱼 Clarias gariepinus 幼鱼 Ca2+-ATPase 和 Na+/K+-ATPase 活性的组织特异性变化

本研究调查了亚致死浓度（0.0、0.3、0.6 和 1.2 mg·L-1）的除草剂恶草酮 (ODZ) 对幼体 Clarias gariepinus 中 Ca2+-ATPase 和 Na+/K+-ATPase 活性的影响。将 180 只 Clarias gariepinus 幼鱼（平均体重 58.88 ± 1.24 g，平均长度 22.34 ± 1.52 cm）随机分为四组，分别暴露于亚致死浓度（0.00、0.3、0.6 和 1.2 mg·L-1 ODZ）在静态更新生物测定系统中持续 21 天，其中每天完全更换除草剂和水。在第 1、7、14 和 21 天测定了鱼鳃、肾脏、肝脏和心脏中 Ca2+- 和 Na+/K+-ATPase 活性的变化。结果显示这些膜-的活性发生了显着变化。结合酶。持续时间和浓度依赖性显着（p < 0. 05) 与对照组相比，处理组的 Ca2+-ATPase 活性增加。与对照相比，Na+/K+-ATPase 活性在所有组织中均被显着抑制（p < 0.05）。在本研究中观察到的 Ca2+-ATPase 和 Na+/K+-ATPase 活性的变化可能表明鱼的离子运输受损和不平衡，这可能引发其他生化、生理甚至神经学后果，从而可能损害多种身体功能. ODZ 对 C. gariepinus ATPase 活性的改变可能会影响鱼体内 ATP 的使用和能量代谢 对 Ca2+ 稳态、Na+/K+ 钠泵和 Ca2+/Na+ 交换器产生严重影响 结果表明酶的测定可作为水污染的生物标志物。