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Carbon nanotube filler enhances incinerated thermoplastics-induced cytotoxicity and metabolic disruption in vitro.
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2020-08-12 , DOI: 10.1186/s12989-020-00371-1 Jayme P Coyle 1 , Raymond C Derk 1 , Tiffany G Kornberg 1, 2 , Dilpreet Singh 3 , Jake Jensen 1 , Sherri Friend 1 , Robert Mercer 1 , Todd A Stueckle 1 , Philip Demokritou 3 , Yon Rojanasakul 2 , Liying W Rojanasakul 1
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2020-08-12 , DOI: 10.1186/s12989-020-00371-1 Jayme P Coyle 1 , Raymond C Derk 1 , Tiffany G Kornberg 1, 2 , Dilpreet Singh 3 , Jake Jensen 1 , Sherri Friend 1 , Robert Mercer 1 , Todd A Stueckle 1 , Philip Demokritou 3 , Yon Rojanasakul 2 , Liying W Rojanasakul 1
Affiliation
Engineered nanomaterials are increasingly being incorporated into synthetic materials as fillers and additives. The potential pathological effects of end-of-lifecycle recycling and disposal of virgin and nano-enabled composites have not been adequately addressed, particularly following incineration. The current investigation aims to characterize the cytotoxicity of incinerated virgin thermoplastics vs. incinerated nano-enabled thermoplastic composites on two in vitro pulmonary models. Ultrafine particles released from thermally decomposed virgin polycarbonate or polyurethane, and their carbon nanotube (CNT)-enabled composites were collected and used for acute in vitro exposure to primary human small airway epithelial cell (pSAEC) and human bronchial epithelial cell (Beas-2B) models. Post-exposure, both cell lines were assessed for cytotoxicity, proliferative capacity, intracellular ROS generation, genotoxicity, and mitochondrial membrane potential. The treated Beas-2B cells demonstrated significant dose-dependent cellular responses, as well as parent matrix-dependent and CNT-dependent sensitivity. Cytotoxicity, enhancement in reactive oxygen species, and dissipation of ΔΨm caused by incinerated polycarbonate were significantly more potent than polyurethane analogues, and CNT filler enhanced the cellular responses compared to the incinerated parent particles. Such effects observed in Beas-2B were generally higher in magnitude compared to pSAEC at treatments examined, which was likely attributable to differences in respective lung cell types. Whilst the effect of the treatments on the distal respiratory airway epithelia remains limited in interpretation, the current in vitro respiratory bronchial epithelia model demonstrated profound sensitivity to the test particles at depositional doses relevant for occupational cohorts.
中文翻译:
碳纳米管填料增强了焚化的热塑性塑料在体外引起的细胞毒性和代谢破坏。
工程纳米材料越来越多地被用作填充剂和添加剂的合成材料。未充分解决生命周期结束后回收和处女和纳米复合材料的潜在病理影响,特别是在焚烧之后。目前的研究旨在在两个体外肺模型上表征焚化的原始热塑性塑料与焚化的纳米级热塑性复合材料的细胞毒性。收集从热分解的原始聚碳酸酯或聚氨酯及其碳纳米管(CNT)复合材料释放的超细颗粒,并将其用于体外急性暴露于原代人小气道上皮细胞(pSAEC)和人支气管上皮细胞(Beas-2B)楷模。暴露后,评估两种细胞系的细胞毒性,增殖能力,细胞内ROS的产生,遗传毒性和线粒体膜电位。处理过的Beas-2B细胞表现出显着的剂量依赖性细胞反应,以及亲本基质依赖性和CNT依赖性敏感性。焚化的聚碳酸酯引起的细胞毒性,活性氧种类的增加和Δm的耗散比聚氨酯类似物显着更有效,并且与焚化的母体颗粒相比,CNT填料增强了细胞反应。在Beas-2B中观察到的这种效应通常比在检查的治疗中的pSAEC更高,这可能归因于各个肺细胞类型的差异。尽管治疗对远端呼吸道上皮细胞的影响在解释上仍然有限,
更新日期:2020-08-12
中文翻译:
碳纳米管填料增强了焚化的热塑性塑料在体外引起的细胞毒性和代谢破坏。
工程纳米材料越来越多地被用作填充剂和添加剂的合成材料。未充分解决生命周期结束后回收和处女和纳米复合材料的潜在病理影响,特别是在焚烧之后。目前的研究旨在在两个体外肺模型上表征焚化的原始热塑性塑料与焚化的纳米级热塑性复合材料的细胞毒性。收集从热分解的原始聚碳酸酯或聚氨酯及其碳纳米管(CNT)复合材料释放的超细颗粒,并将其用于体外急性暴露于原代人小气道上皮细胞(pSAEC)和人支气管上皮细胞(Beas-2B)楷模。暴露后,评估两种细胞系的细胞毒性,增殖能力,细胞内ROS的产生,遗传毒性和线粒体膜电位。处理过的Beas-2B细胞表现出显着的剂量依赖性细胞反应,以及亲本基质依赖性和CNT依赖性敏感性。焚化的聚碳酸酯引起的细胞毒性,活性氧种类的增加和Δm的耗散比聚氨酯类似物显着更有效,并且与焚化的母体颗粒相比,CNT填料增强了细胞反应。在Beas-2B中观察到的这种效应通常比在检查的治疗中的pSAEC更高,这可能归因于各个肺细胞类型的差异。尽管治疗对远端呼吸道上皮细胞的影响在解释上仍然有限,