The amount of copper affects the quality of herbal medicine greatly, it is necessary to develop some simple and sensitive methods to detect copper for the remote or resource-limited area. An electronic balance is one of the most familiar equipment that can be found nearly in all laboratories. The presence of Cu(I) can catalyze azide–alkyne cycloaddition reaction (called as click chemistry) with high efficiency. In this study, a simple method had been developed to detect copper ions in herbal medicine using an electronic balance as a readout device based on click chemistry. Cu(II) is reduced to Cu(I) by sodium ascorbate in situ, which induces the “click” reaction between azido–DNA modified magnetic beads (MB–DNA) and alkynyl–DNA modified platinum nanoparticles (Pt NP–DNA) and results in the fixing of the platinum nanoparticles on the beads (called as MB–Pt NPs). MB–Pt NPs can be separated by a magnetic frame easily and transferred into a drainage reaction device containing hydrogen peroxide. Then, hydrogen peroxide can be decomposed by Pt NPs modified on MB to generate oxygen, which increases the pressure in the drainage reaction device and forces the water in the system to be discharged. The weight of the discharged water can be easily and accurately measured by an electronic balance. The weight of the water has a linear relationship with Cu(II) in the range of 2.0–200 μM and a detection limit of 0.83 μM under 30 min of collected time. This method does not need complicated and expensive instruments, skilled technicians, and a complex data processing process. The proposed method had been applied to detect copper ions in herbal medicine with satisfactory results.

中文翻译：

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使用电子天平作为读数，基于点击化学测定草药中的铜离子。

铜的含量对草药的质量影响很大，有必要开发一些简单而灵敏的方法来检测偏远或资源有限地区的铜。电子天平是几乎在所有实验室中都能找到的最熟悉的设备之一。Cu（I）的存在可以高效催化叠氮化物-炔烃的环加成反应（称为点击化学）。在这项研究中，已经开发了一种简单的方法，可以使用电子天平作为基于点击化学的读出设备来检测草药中的铜离子。原位通过抗坏血酸钠将Cu（II）还原为Cu（I），它诱导叠氮基– DNA修饰的磁珠（MB–DNA）与炔基–DNA修饰的铂纳米颗粒（Pt NP–DNA）之间的“喀哒”反应，并导致铂纳米颗粒固定在珠上（称为MB– Pt NPs）。MB-Pt NPs可以很容易地被磁性框架分离，然后转移到含有过氧化氢的排水反应装置中。然后，过氧化氢可以通过修饰在MB上的Pt NPs分解而产生氧气，这会增加排水反应装置中的压力并迫使系统中的水排出。排放的水的重量可以通过电子天平轻松而准确地测量。水的重量与Cu（II）的收集时间为30分钟（2.0-200μM，检测极限为0.83μM）。该方法不需要复杂且昂贵的仪器，熟练的技术人员以及复杂的数据处理过程。该方法已用于中草药中铜离子的检测，结果令人满意。