The adapted industrial yeast strain Saccharomyces cerevisiae NRRL Y-50049 is able to in situ detoxify major toxic aldehyde compounds derived from sugar conversion of lignocellulosic biomass while producing ethanol. Pathway-based studies on its mechanisms of tolerance have been reported previously, however, little is known about transposable element (TE) involvement in its adaptation to inhibitory compounds. This work presents a comparative dynamic transcription expression analysis in response to a toxic treatment between Y-50049 and its progenitor, an industrial type strain NRRL Y-12632, using a time-course study. At least 77 TEs from Y-50049 showed significantly increased expression compared with its progenitor, especially during the late lag phase. Sequence analysis revealed significant differences in TE sequences between the two strains. Y-50049 was also found to have a transposons of yeast 2 (Ty2) long terminal repeat-linked YAT1 gene showing significantly higher copy number changes than its progenitor. These results raise awareness of potential TE involvement in the adaptation of industrial yeast to the tolerance of toxic chemicals.

中文翻译：

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潜在转座因子对工业酵母酿酒酵母适应性影响的一瞥。

适应的工业酵母菌株酿酒酵母NRRL Y-50049 能够原位在生产乙醇的同时，对木质纤维素生物质的糖转化产生的主要有毒醛化合物进行解毒。先前已经报道了对其耐受机制的基于途径的研究，但是，关于转座因子 (TE) 参与其对抑制性化合物的适应知之甚少。这项工作使用时间进程研究，对 Y-50049 及其祖先工业型菌株 NRRL Y-12632 之间的毒性处理进行了比较动态转录表达分析。与它的祖先相比，来自 Y-50049 的至少 77 个 TE 显示出显着增加的表达，尤其是在晚期滞后阶段。序列分析揭示了两个菌株之间TE序列的显着差异。还发现 Y-50049 具有酵母 2 (Ty2) 长末端重复连接的转座子YAT1基因显示出显着高于其祖先的拷贝数变化。这些结果提高了人们对潜在 TE 参与使工业酵母适应有毒化学品耐受性的认识。