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Oxygen Isotope Analysis of Saline Solutions by a Carbonic anhydrase-Catalyzed CO2–H2O Equilibration Method (C3HEM) with an Improved Drying Technique
ACS Earth and Space Chemistry ( IF 3.4 ) Pub Date : 2020-08-12 , DOI: 10.1021/acsearthspacechem.0c00143
Sang-Tae Kim 1 , Martin Knyf 1
Affiliation  

The recently introduced carbonic anhydrase-catalyzed CO2–H2O equilibration method (or C3HEM) for oxygen isotopes of aqueous samples has remarkably improved the analytical efficiency of the classic and most popular stable isotope analytical method in geochemistry. However, the applicability of this novel analytical method has not yet been tested for saline/hypersaline solutions despite their significance in various scientific problems. In this study, 1 m chloride and sulfate solutions of geochemical importance were used to investigate the kinetics of oxygen isotope exchange between CO2 and H2O at 25 °C. For these saline solutions, oxygen isotope equilibrium times (EQTs), rate constants, and oxygen isotope salt effects (OISEs) were determined with 4 or 16 μm carbonic anhydrase (CA). Our experimental results reveal that EQTs, ranging from 0.73 to 4.48 h, were much shorter than the typical EQT, confirming the greatly improved analytical efficiency of the C3HEM for saline solutions. In addition, a new simpler CA drying technique was developed, further improving the analytical efficiency of the C3HEM by significantly reducing the required labor time for sample vial preparation. Detailed analytical protocols and practical guidelines of the C3HEM are provided for prospective users.

中文翻译:

碳酸酐酶催化的CO 2 -H 2 O平衡法(C 3 HEM)和改进的干燥技术对盐溶液的氧同位素分析

最近引入的碳酸酐酶催化的CO 2 -H 2 O平衡方法(或C 3 HEM)用于水性样品的氧同位素,显着提高了地球化学中经典且最受欢迎的稳定同位素分析方法的分析效率。然而,尽管这种新颖的分析方法在各种科学问题中具有重要意义,但尚未针对盐/高盐溶液进行测试。在这项研究中,使用具有地球化学重要性的1 m氯化物和硫酸盐溶液研究CO 2和H 2之间的氧同位素交换动力学在25°C下为O。对于这些盐水溶液,氧同位素平衡时间(EQTs),速率常数和氧同位素的盐效应(OISEs)用4或16μ确定中号碳酸酐酶(CA)。我们的实验结果表明,EQT的范围从0.73到4.48小时,比典型的EQT短得多,这证实了C 3 HEM对盐溶液的分析效率大大提高了。另外,开发了一种新的更简单的CA干燥技术,通过显着减少样品瓶制备所需的劳动时间,进一步提高了C 3 HEM的分析效率。为潜在用户提供了C 3 HEM的详细分析协议和实用准则。
更新日期:2020-09-18
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