Multistimuli-responsive nanomedicines present great potential for cancer therapy, as they can be featured as simple, selective, and smart carriers that can release their payload on-demand. In this study, we prepared a multifunctional polymeric vesicular nanocarrier (PVN) based on robust and triple stimuli-responsive micelles that could encapsulate chemotherapeutic drugs (doxorubicin (DOX)) and photothermal agents (IR780 iodide) for combined chemo-photothermal therapy. The size of the PVNs was stable and uniform (∼100 nm), and its DOX and IR780 loading were high: 26.5 and 16.4 wt %, respectively. Further in vitro investigations suggested that the DOX/IR780 coloaded PVNs presented controlled drug release kinetics upon costimulation with specific endogenous stimuli. Upon laser irradiation, DOX/IR780 coloaded PVNs exhibited prominent photothermal cytotoxicity toward murine colon cancer (CT-26) cells. Intracellular uptake assays indicated that DOX/IR780 coloaded PVNs could be readily uptaken by CT-26 cells, resulting in the release of DOX within the cytoplasm of the cells in response to laser irradiation.

中文翻译：

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在化学光热疗法中加速药物释放的多刺激响应性聚合物囊泡。

对多种刺激有反应的纳米药物具有巨大的癌症治疗潜力，因为它们具有简单，选择性和智能的特点，可以按需释放有效载荷。在这项研究中，我们基于强大和三重刺激反应性胶束制备了多功能聚合物水泡纳米载体（PVN），该胶束可以封装化疗药物（阿霉素（DOX））和光热剂（IR780碘化物）以进行化学光热疗法联合治疗。PVN的尺寸稳定且均匀（约100 nm），其DOX和IR780的负载较高：分别为26.5 wt％和16.4 wt％。进一步体外研究表明，在共刺激特定内源性刺激后，DOX / IR780共载PVN呈现出受控的药物释放动力学。在激光照射下，DOX / IR780共负载的PVN对鼠结肠癌（CT-26）细胞表现出显着的光热细胞毒性。细胞内吸收测定表明，DO-X / IR780担载的PVNs可以很容易地被CT-26细胞吸收，从而导致DOX在细胞质内释放，以响应激光照射。