Abstract

Background

Acute pancreatitis (AP) associated with the modulation of TNF-α-AMPK axis in the presence and absence of vitamin E has not been investigated before.

Material and methods

Rats were either injected with L-arginine (2.5 gm/kg) before being sacrificed after 48 h or were pre-treated with vitamin E (60 mg/kg) and continued receiving vitamin E until the end of the experiment.

Results

AP was developed as demonstrated by infiltration of inflammatory cells and profound pancreas tissue damage, which were substantially protected by vitamin E. In addition, L-arginine injections significantly (p < .0001) increased the expression of TNF-α mRNA and protein, and decreased phospho-AMPK and IL-10 mRNA and protein that was significantly (p < .0001) protected by vitamin E. Furthermore, vitamin E inhibited L-arginine-induced blood levels of LDH, amylase, and myeloperoxidase.

Conclusions

L-arginine-induced acute pancreatitis modulates TNF-α-AMPK axis, IL-10 and other AP biomarkers, which is protected by vitamin E; thus, may offer therapeutic potential in humans.

中文翻译：

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维生素 E 在 L-精氨酸诱导的急性坏死性胰腺炎大鼠模型中防止 TNF-α-AMPK 轴的调节并抑制胰腺损伤

摘要

背景

之前没有研究过在存在和不存在维生素 E 的情况下与 TNF-α-AMPK 轴调节相关的急性胰腺炎 (AP)。

材料与方法

大鼠要么在 48 小时后处死前注射 L-精氨酸 (2.5 gm/kg)，要么用维生素 E (60 mg/kg) 预处理并继续接受维生素 E 直至实验结束。

结果

AP 的发展如炎症细胞浸润和严重胰腺组织损伤所证明的那样，这些组织受到维生素 E 的充分保护。此外，L-精氨酸注射显着 ( p  < .0001) 增加了 TNF-α mRNA 和蛋白质的表达，并且减少受维生素 E 显着 ( p  < .0001) 保护的磷酸化 AMPK 和 IL-10 mRNA 和蛋白质。此外，维生素 E 抑制 L-精氨酸诱导的 LDH、淀粉酶和髓过氧化物酶的血液水平。

结论

L-精氨酸诱导的急性胰腺炎调节 TNF-α-AMPK 轴、IL-10 和其他受维生素 E 保护的 AP 生物标志物；因此，可能为人类提供治疗潜力。