Abstract The objective of this work was to develop a subgingival biofilm model using a stirred bioreactor. Discs of bovine teeth were adapted to a stirred bioreactor filled with a culture medium containing bacterial species associated with periodontal health or disease. After anaerobic incubation, the biofilms growing on the substratum surfaces were collected and analyzed. The mean number of Colony-forming Units (CFUs) varied, but with no difference between 3 and 7 days of biofilm formation (p > 0.05). Scanning Electron Microscopy (SEM) analysis showed a uniform biofilm layer covering the cement layer of the root surface containing bacteria with diverse morphology. In checkerboard DNA-DNA hybridization, bacterial species were identified in both biofilms. In conclusion, a subgingival biofilm model was developed using a stirred bioreactor, allowing the in vitro reproduction of complex microbial communities. This is an advanced model that may be useful to mimic complex clinical periodontal biofilms.

中文翻译：

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在搅拌生物反应器中开发多物种牙周生物膜模型

摘要 这项工作的目的是开发使用搅拌生物反应器的龈下生物膜模型。牛牙盘适应搅拌生物反应器，该生物反应器装有含有与牙周健康或疾病相关的细菌种类的培养基。厌氧培养后，收集并分析生长在基质表面的生物膜。菌落形成单位 (CFU) 的平均数量有所不同，但在生物膜形成的 3 天和 7 天之间没有差异 (p > 0.05)。扫描电子显微镜 (SEM) 分析显示均匀的生物膜层覆盖根表面的水泥层，其中含有形态各异的细菌。在棋盘 DNA-DNA 杂交中，在两种生物膜中都鉴定了细菌种类。总之，使用搅拌生物反应器开发了龈下生物膜模型，允许复杂微生物群落的体外繁殖。这是一种先进的模型，可用于模拟复杂的临床牙周生物膜。