Vine pruning residues are by-products of the wine industry that have not received much attention in the past, in spite of being rich in bioactive compounds. In this study, we aimed to test whether an ohmic extract of vine pruning residue (VPE) has anti-colorectal cancer (CRC) properties, and whether responses differ according with cell’s mutation profile. VPE decreased human CRC cell proliferation, accompanied by DNA effects and cell cycle modulation. VPE also increased cell sensitivity to the chemotherapeutic drug 5-FU. Our results suggest that tumors harboring BRAF mutations may be more responsive to VPE than KRAS mutated tumors. These effects of the extract were not completely reproduced by the most abundant constituents tested individually at the concentrations present in the effective dose of VPE. Globally, our results indicate that VPE, a polyphenol enriched extract produced by ohmic heating of vine pruning residue, has anti-colorectal cancer potential, including sensitizing to a chemotherapeutical drug, and its use in functional foods or nutraceuticals could be exploited in personalized anti colorectal cancer dietary strategies. Valorization of this lignocellulosic residue should encourage bio-waste recycling, adding value to this agricultural by-product and promoting the sustainable use of natural resources.

中文翻译：

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藤枝修剪残渣的欧姆加热提取物具有抗结直肠癌的活性，并提高了对化学治疗药物5-FU的敏感性。

藤修剪残留物是葡萄酒行业的副产品，尽管富含生物活性化合物，但过去并未受到太多关注。在这项研究中，我们旨在测试藤枝修剪残留物（VPE）的欧姆提取物是否具有抗结肠直肠癌（CRC）的特性，以及响应是否根据细胞的突变情况而有所不同。VPE降低了人类CRC细胞的增殖，并伴有DNA效应和细胞周期调控。VPE还增加了对化学治疗药物5-FU的细胞敏感性。我们的结果表明，携带BRAF突变的肿瘤对VPE的反应可能比对KRAS突变的肿瘤更敏感。在有效剂量的VPE中，单独测试的最丰富的成分并未完全再现提取物的这些作用。在全球范围内，我们的结果表明，VPE，通过对藤修剪残渣进行欧姆加热产生的富含多酚的提取物，具有抗结直肠癌的潜力，包括对化学药物敏感，并且可以在个性化的抗结直肠癌饮食策略中开发其在功能性食品或保健食品中的用途。木质纤维素残留物的增值应鼓励生物废物的循环利用，为这种农业副产品增加价值并促进自然资源的可持续利用。