The Brassicaceae consists of a wide range of species, including important Brassica crop species and the model plant Arabidopsis (Arabidopsis thaliana). Brassica spp. crop diseases impose significant yield losses annually. A major way to reduce susceptibility to disease is the selection in breeding for resistance gene analogs (RGAs). Nucleotide binding site-leucine rich repeats (NLRs), receptor-like kinases (RLKs), and receptor-like proteins (RLPs) are the main types of RGAs; they contain conserved domains and motifs and play specific roles in resistance to pathogens. Here, all classes of RGAs have been identified using annotation and assembly-based pipelines in all available genome annotations from the Brassicaceae, including multiple genome assemblies of the same species where available (total of 32 genomes). The number of RGAs, based on genome annotations, varies within and between species. In total 34,065 RGAs were identified, with the majority being RLKs (21,691), then NLRs (8,588) and RLPs (3,786). Analysis of the RGA protein sequences revealed a high level of sequence identity, whereby 99.43% of RGAs fell into several orthogroups. This study establishes a resource for the identification and characterization of RGAs in the Brassicaceae and provides a framework for further studies of RGAs for an ultimate goal of assisting breeders in improving resistance to plant disease.

十字花科由多种物种组成，包括重要的芸苔属作物物种和模式植物拟南芥（Arabidopsis thaliana）。芸苔属 农作物病害每年都会造成严重的产量损失。降低疾病易感性的一个主要方法是在育种中选择抗性基因类似物（RGAs）。核苷酸结合位点富含亮氨酸重复序列（NLR）、类受体激酶（RLK）和类受体蛋白（RLP）是RGA的主要类型；它们包含保守的结构域和基序，并在抵抗病原体方面发挥特定作用。在这里，所有类别的 RGA 均已使用十字花科所有可用基因组注释中的注释和基于组装的管道进行了识别，包括可用的同一物种的多个基因组组装（总共 32 个基因组）。根据基因组注释，RGA 的数量在物种内部和物种之间存在差异。总共鉴定了 34,065 个 RGA，其中大多数是 RLK（21,691），然后是 NLR（8,588）和 RLP（3,786）。对 RGA 蛋白序列的分析揭示了高水平的序列同一性，其中 99.43% 的 RGA 属于多个正交群。这项研究为十字花科植物中 RGA 的鉴定和表征建立了资源，并为 RGA 的进一步研究提供了框架，以实现协助育种者提高植物抗病性的最终目标。