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Regucalcin enhances adipocyte differentiation and attenuates inflammation in 3T3-L1 cells.
FEBS Open Bio ( IF 2.6 ) Pub Date : 2020-08-11 , DOI: 10.1002/2211-5463.12947
Tomiyasu Murata 1 , Masayoshi Yamaguchi 2 , Susumu Kohno 3 , Chiaki Takahashi 3 , Watanabe Risa 1 , Kanna Hatori 1 , Kiyomi Hikita 1 , Norio Kaneda 1
Affiliation  

Dysregulation of adipocyte differentiation and dysfunction play key roles in the pathogenesis of obesity and associated disorders such as diabetes and metabolic syndrome, and as such, a better understanding of the molecular mechanism of adipogenesis may help to elucidate the pathological condition of obesity and its associated disorders. Regucalcin (RGN) plays multiple regulatory roles in intracellular Ca2+ signaling pathways in mammalian cells. Here, we report that overexpression of RGN enhances lipid accumulation in 3T3‐L1 adipocyte cells after adipogenic stimulation, accompanied by upregulation of adipocyte differentiation marker proteins. In contrast, genetic disruption of RGN inhibited adipogenic stimulation‐induced differentiation of 3T3‐L1 cells. Furthermore, RGN overexpression in differentiated 3T3‐L1 adipocytes blocked inflammatory crosstalk between 3T3‐L1 adipocytes and RAW264.7 macrophages in a transwell coculture system. Knockdown of RGN expression in cocultured 3T3‐L1 adipocytes enhanced their susceptibility to RAW264.7 macrophage‐mediated inflammation. These results suggest that RGN is required for 3T3‐L1 adipocyte differentiation and that it exerts anti‐inflammatory activity against 3T3‐L1 adipocyte inflammation after coculture with RAW264.7 macrophages. Thus, RGN may be a novel regulator of adipocyte differentiation and act as a suppressor of inflammation in macrophage‐infiltrated adipocyte tissue.

中文翻译:

Regucalcin 增强脂肪细胞分化并减轻 3T3-L1 细胞中的炎症。

脂肪细胞分化失调和功能障碍在肥胖和相关疾病(如糖尿病和代谢综合征)的发病机制中起关键作用,因此,更好地了解脂肪生成的分子机制可能有助于阐明肥胖及其相关疾病的病理状况. Regucalcin (RGN) 在细胞内 Ca 2+中发挥多种调节作用哺乳动物细胞中的信号通路。在这里,我们报告说,RGN 的过表达增强了脂肪形成刺激后 3T3-L1 脂肪细胞中的脂质积累,并伴随脂肪细胞分化标记蛋白的上调。相比之下,RGN 的遗传破坏抑制了脂肪刺激诱导的 3T3-L1 细胞分化。此外,分化的 3T3-L1 脂肪细胞中的 RGN 过表达阻断了 transwell 共培养系统中 3T3-L1 脂肪细胞和 RAW264.7 巨噬细胞之间的炎症串扰。共培养的 3T3-L1 脂肪细胞中 RGN​​ 表达的敲低增强了它们对 RAW264.7 巨噬细胞介导的炎症的易感性。这些结果表明,RGN 是 3T3-L1 脂肪细胞分化所必需的,并且在与 RAW264.7 巨噬细胞共培养后,它对 3T3-L1 脂肪细胞炎症具有抗炎活性。因此,RGN 可能是一种新的脂肪细胞分化调节剂,并在巨噬细胞浸润的脂肪细胞组织中起到抑制炎症的作用。
更新日期:2020-10-02
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