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3D mesoporous bioactive glass/silk/chitosan scaffolds and their compatibility with human adipose‐derived stromal cells
International Journal of Applied Ceramic Technology ( IF 2.1 ) Pub Date : 2020-08-11 , DOI: 10.1111/ijac.13607
Ratiya Buapa Phetnin 1 , Sanong Suksaweang 2 , Chiara Giannasi 3 , Anna Teresa Brini 3, 4 , Stefania Niada 3 , Sawitri Srisuwan 1 , Sirirat Tubsungnoen Rattanachan 1
Affiliation  

Human adipose‐derived stem/stromal cells (hASCs) have been popularly studied as cell‐based therapy in the field of regenerative medicine due to their ability to differentiate into several cell types. In this study, in order to improve the mechanical strength and bioactivity of scaffolds for bone tissue engineering, three types of mesoporous bioactive glasses with different shapes and compositions were dispersed in the silk fibroin/chitosan (SF/CS)‐based scaffolds, which were fabricated with a combination of freezing and lyophilization. The characteristic and physical properties of these composite scaffolds were evaluated. The biocompatibility was also assessed through hASCs in vitro tests. Both Alamar Blue® and Live/Dead assay® revealed that the spherical mesoporous bioactive glass doped scaffolds enhanced cell viability and proliferation. Furthermore, the addition of spherical mesoporous bioactive glass into SF/CS scaffolds encouraged hASC osteogenic differentiation as well. These results suggested that this composite scaffold can be applicable material for bone regeneration.

中文翻译:

3D介孔生物活性玻璃/丝绸/壳聚糖支架及其与人类脂肪基质细胞的相容性

人类脂肪干细胞/基质细胞(hASC)由于能够分化为几种细胞类型,因此在再生医学领域已被广泛研究为基于细胞的疗法。在这项研究中,为了提高用于骨组织工程的支架的机械强度和生物活性,将三种不同形状和成分的中孔生物活性玻璃分散在基于丝素蛋白/壳聚糖(SF / CS)的支架中,分别是通过冷冻和冻干的组合制成。评价了这些复合支架的特征和物理性质。还通过hASCs体外测试评估了生物相容性。AlamarBlue®和Live / Deadassay®均表明,球形介孔生物活性玻璃掺杂支架可增强细胞活力和增殖。此外,向SF / CS支架中添加球形中孔生物活性玻璃也促进了hASC的成骨分化。这些结果表明该复合支架可以作为骨再生的适用材料。
更新日期:2020-10-05
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