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The mechanism of damage to the posterior silk gland by trace amounts of acetamiprid in the silkworm, Bombyx mori
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.pestbp.2020.104676
Zhengting Lu , Mengxue Li , Yilong Fang , Jianwei Qu , Wentao Ye , Minli Dai , Dandan Bian , Tingting Mao , Fanchi Li , Haina Sun , Bing Li

Acetamiprid is a new neonicotinoid insecticide widely used in the prevention and control of pests in agriculture. However, its residues in the environment affect the cocooning of the silkworm, Bombyx mori (B. mori), a non-target insect. To investigate the mechanism of damage, B. mori larvae were fed with trace amounts of acetamiprid (0.15 mg/L). At 96 h after exposure, the larvae showed signs of poisoning and decreased body weight, resulting in reduced survival and ratio of cocoon shell. At 48 h and 96 h after exposure, the residues in the posterior silk gland (PSG), which is responsible for synthesizing silk fibroin, were 0.72 μg/mg and 1.21 μg/mg, respectively, as measured by high performance liquid chromatography, indicating that acetamiprid can accumulate in the PSG. Moreover, pathological sections and transmission electron microscopy also demonstrate the damage of the PSG by acetamiprid. Digital gene expression (DGE) and KEGG pathway enrichment analysis revealed that genes related to metabolism, stress responses and inflammation were significantly up-regulated after exposure. Quantitative RT-PCR analysis showed that the transcript levels of FMBP-1 and FTZ-F1 (transcription factors for synthesizing silk protein) were up-regulated by 2.55-and 1.56-fold, respectively, and the transcript levels of fibroin heavy chain (Fib-H), fibroin light chain (Fib-L), P25, Bmsage and Bmdimm were down-regulated by 0.75-, 0.76-, 0.65-, 0.44- and 0.40-fold, respectively. The results indicate that accumulated acetamiprid causes damage to the PSG and leads to reduced expression of genes responsible for synthesizing silk fibroin. Our data provide reference for evaluating the safety of acetamiprid residues in the environment for non-target insects.

中文翻译:

家蚕Bombyx mori中微量啶虫脒损伤后丝腺的机制

啶虫脒是一种新型的新烟碱类杀虫剂,广泛用于农业害虫的防治。然而,它在环境中的残留会影响非目标昆虫家蚕 Bombyx mori (B. mori) 的结茧。为了研究损伤机制,给家蚕幼虫喂食微量啶虫脒(0.15 毫克/升)。暴露后96小时,幼虫出现中毒迹象,体重下降,导致成活率和茧壳比例下降。在暴露后 48 小时和 96 小时,负责合成丝素蛋白的后部丝腺 (PSG) 中的残留物通过高效液相色谱法测量分别为 0.72 μg/mg 和 1.21 μg/mg,表明啶虫脒可以在 PSG 中积累。而且,病理切片和透射电子显微镜也证明了啶虫脒对 PSG 的损伤。数字基因表达(DGE)和KEGG通路富集分析表明,暴露后与代谢、应激反应和炎症相关的基因显着上调。定量 RT-PCR 分析表明,FMBP-1 和 FTZ-F1(合成丝蛋白的转录因子)的转录水平分别上调了 2.55 和 1.56 倍,丝心蛋白重链(Fib -H)、丝心蛋白轻链 (Fib-L)、P25、Bmsage 和 Bmdimm 分别下调 0.75、0.76、0.65、0.44 和 0.40 倍。结果表明,积累的啶虫脒对 PSG 造成损害,并导致负责合成丝素蛋白的基因表达减少。
更新日期:2020-11-01
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