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Resistance to pyridaben in Canadian greenhouse populations of two-spotted spider mites, Tetranychus urticae (Koch)
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.pestbp.2020.104677
Hooman Hosseinzadeh Namin , Vladimir Zhurov , Jeremy Spenler , Miodrag Grbić , Vojislava Grbić , Ian M. Scott

Two-spotted spider mite (TSSM) Tetranychus urticae (Koch) is an important agricultural pest that causes considerable yield losses to over 150 field and greenhouse crops. Mitochondrial electron transport inhibitors (METI) acaricides are commonly used to control mite species in commercial Canadian greenhouses. Development of resistance to METIs in TSSM populations have been reported worldwide, but not until recently in Canada. The objectives of this study were to: 1) monitor the acaricide-susceptibility in greenhouse TSSM populations, and 2) investigate the resistance to pyridaben, a METI acaricide, in greenhouse resistant and pyridaben-selected (SRS) mite strains. The increased mortality to the pyridaben sub-lethal concentration (LC30) when SRS mites were exposed to piperonyl butoxide (PBO), a general cytochrome P450 monooxygenase inhibitor, and higher P450 activity compared to the greenhouse strain (RS) mites, indicated that P450s may be at least partially responsible for the resistance. The molecular mechanisms of target site insensitivity-mediated resistance in the pyridaben resistant strain of TSSM were investigated by comparing the DNA sequence of NADH dehydrogenase subunits TYKY and PSST, NADH-ubiquinone oxidoreductase chain 1 and 5 (ND1, ND5) and the NADH-ubiquinone oxidoreductase subunit 49 kDa from SRS to the reference strain (SS) and RS. Despite a number of nucleotide substitutions, none correlated with the pyridaben resistance. Understanding the underlying mechanisms of TSSM adaptation to acaricides is an essential part of resistance management strategy in any IPM program. The findings of this study will encourage growers to apply acaricides with different modes of action to reduce the rate at which acaricide resistance will occur in greenhouse TSSM populations.

中文翻译:

加拿大温室二斑叶螨(Tetranychus urticae (Koch))种群对哒螨灵的抗性

二斑叶螨 (TSSM) Tetranychus urticae (Koch) 是一种重要的农业害虫,对 150 多种大田和温室作物造成相当大的产量损失。线粒体电子传递抑制剂 (METI) 杀螨剂通常用于控制加拿大商业温室中的螨类。TSSM 人群对 METI 产生耐药性的报道已在世界范围内报道,但直到最近才在加拿大报道。本研究的目的是:1) 监测温室 TSSM 种群中的杀螨剂敏感性,以及 2) 调查温室抗性和杀螨剂选择 (SRS) 螨菌株对杀螨剂(一种 METI 杀螨剂)的抗性。当 SRS 螨暴露于胡椒基丁醚 (PBO)(一种通用的细胞色素 P450 单加氧酶抑制剂)时,杀虫剂亚致死浓度 (LC30) 的死亡率增加,与温室菌株 (RS) 螨相比,P450 活性更高,表明 P450 可能至少部分是造成抗性的原因。通过比较NADH脱氢酶亚基TYKY和PSST、NADH-泛醌氧化还原酶链1和5(ND1、ND5)和NADH-泛醌的DNA序列,研究了TSSM抗哒嗪菌中靶位点不敏感性介导的抗性的分子机制。氧化还原酶亚基 49 kDa 从 SRS 到参考菌株 (SS) 和 RS。尽管有许多核苷酸取代,但没有一个与哒螨灵抗性相关。了解 TSSM 适应杀螨剂的潜在机制是任何 IPM 计划中抗性管理策略的重要组成部分。
更新日期:2020-11-01
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