Journal of Microbiology, Immunology and Infection ( IF 7.4 ) Pub Date : 2020-08-12 , DOI: 10.1016/j.jmii.2020.07.020 Cheng-Chun Tai , Cheng-Hsien Tsai , Yu-Han Huang , Chia-Lin Lee , Hsin-Pai Chen , Yu-Jiun Chan
Background
Respiratory viruses (RVs) are among the most common pathogens for both upper and lower respiratory tract infections (RTIs). However, the viral epidemiology of RV-associated RTIs in adults has long been under-recognized. Through a sensitive molecular assay, it would be possible to have a better understanding of the epidemiology of RV-associated RTIs.
Material and methods
Respiratory tract (RT) specimens from adults hospitalized due to RTIs were tested for RVs, using the multiplex PCR-based Luminex xTAG® Respiratory Viral Panel assay. A total of nineteen RVs, including influenza viruses and non-influenza respiratory viruses (NIRVs) were detected. Positive rates were compared using a chi-square test.
Results
A total of 2292 samples from adult patients hospitalized with RTIs were screened for RVs. The overall positive rate was 22%, with 17.8% samples positive for at least one NIRV. NIRVs had a higher positive rate in non-winter seasons. As many as 12.7% (46/363) of the samples collected through broncho-alveolar lavage and 20.5% (176/859) of the samples collected in ICUs were positive for RVs. Distribution of corona virus (CoV), human metapneumovirus (hMPV) and parainfluenza virus (PIV) demonstrated seasonal variation. Also, temperature was associated with the positive rates of specific viruses, including CoV, respiratory syncytial virus (RSV), hMPV and PIV.
Conclusion
Respiratory viruses, notably NIRVs, were frequently detected in adults hospitalized with RTIs. Several RVs were detected with distinctive seasonal variations. A substantial number of RVs were identified in lower RT specimens or from patients admitted to ICU, highlighting their important role in causing severe respiratory infection.
中文翻译:
在三级中心使用多重 PCR 检测检测成人呼吸道感染的呼吸道病毒
背景
呼吸道病毒 (RV) 是上呼吸道和下呼吸道感染 (RTI) 最常见的病原体之一。然而,成人 RV 相关 RTI 的病毒流行病学长期以来一直未被充分认识。通过灵敏的分子检测,可以更好地了解 RV 相关 RTI 的流行病学。
材料与方法
使用基于多重 PCR 的 Luminex xTAG® Respiratory Viral Panel 检测对因 RTI 住院的成年人的呼吸道 (RT) 标本进行 RV 检测。共检测到 19 种 RV,包括流感病毒和非流感呼吸道病毒 (NIRV)。使用卡方检验比较阳性率。
结果
共对 2292 份因 RTI 住院的成年患者样本进行了 RV 筛查。总体阳性率为 22%,17.8% 的样本对至少一种 NIRV 呈阳性。NIRV 在非冬季的阳性率更高。多达 12.7% (46/363) 通过支气管肺泡灌洗收集的样本和 20.5% (176/859) 在 ICU 收集的样本对 RV 呈阳性。冠状病毒 (CoV)、人类偏肺病毒 (hMPV) 和副流感病毒 (PIV) 的分布表现出季节性变化。此外,温度与特定病毒的阳性率有关,包括 CoV、呼吸道合胞病毒 (RSV)、hMPV 和 PIV。
结论
呼吸道病毒,尤其是 NIRV,经常在患有 RTIs 的成人中检测到。检测到几个具有明显季节性变化的房车。在较低的放疗样本或入住 ICU 的患者中发现了大量 RV,突出了它们在导致严重呼吸道感染中的重要作用。