Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger (P-Rex) proteins control many fundamental cellular functions including cell migration, actin cytoskeletal rearrangement and adhesion in many cancers. However, P-Rex1 expression and its prognostic effect and possible clinical value are not clearly elucidated in human oral squamous cell carcinoma (OSCC). Here, OSCC tissue microarrays were used to verify the expression levels of P-Rex1, coinhibitory immune checkpoints and tumor associated macrophage (TAM) markers, and to analyze the relationship between PREX1 expression levels and clinicopathological characteristics in OSCC. The study found that P-Rex1 expression was elevated in OSCC compared to dysplasia and normal mucosa (P < 0.0001). In addition, patients who expressed high PREX1 had a poorer prognosis than those who expressed low PREX1 (P = 0.0070). Furthermore, positive correlations were found between P-Rex1 expression and the immune checkpoints PD-L1, Galectin-9 and B7-H4, and the TAM markers CD68, CD206 and CD163. In short, these findings implicated that overexpression of P-Rex1 may predict a poor prognosis in human OSCC.

磷脂酰肌醇3,4,5-三磷酸依赖性Rac交换子（P-Rex）蛋白控制许多细胞的基本细胞功能，包括细胞迁移，肌动蛋白细胞骨架重排和粘附。然而，在人口腔鳞状细胞癌（OSCC）中，P-Rex1的表达及其预后效果和可能的临床价值尚不清楚。在这里，OSCC组织芯片用于验证P-Rex1，共抑制免疫检查点和肿瘤相关巨噬细胞（TAM）标记的表达水平，并分析PRECC1表达水平与OSCC中临床病理特征之间的关系。研究发现，与异型增生和正常粘膜相比，OSCC中的P-Rex1表达升高（P <0.0001）。此外，高PREX1的患者预后较低PREX1的患者差（P  = 0.0070）。此外，在P-Rex1表达与免疫检查点PD-L1，Galectin-9和B7-H4以及TAM标记CD68，CD206和CD163之间发现正相关。简而言之，这些发现暗示P-Rex1的过表达可能预示了人类OSCC的不良预后。