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Elasticity-dependent response of malignant cells to viscous dissipation.
Biomechanics and Modeling in Mechanobiology ( IF 3.5 ) Pub Date : 2020-08-12 , DOI: 10.1007/s10237-020-01374-9
Elisabeth E Charrier 1, 2, 3 , Katarzyna Pogoda 1, 3, 4 , Robin Li 1 , Rebecca G Wells 2, 3 , Paul A Janmey 1, 3, 5
Affiliation  

The stiffness of the cellular environment controls malignant cell phenotype and proliferation. However, the effect of viscous dissipation on these parameters has not yet been investigated, in part due to the lack of in vitro cell substrates reproducing the mechanical properties of normal tissues and tumors. In this article, we use a newly reported viscoelastic polyacrylamide gel cell substrate, and we characterize the impact of viscous dissipation on three malignant cell lines: DU145 and PC3 derived from prostate and LN229 from brain. The spreading, motility and proliferation rates of these cells were analyzed on 1 kPa and 5 kPa elastic and viscoelastic gels. Surprisingly, the effect of substrate viscous dissipation on cell behavior depended on substrate stiffness for the three cell types tested. We conclude that viscoelasticity controls the spreading, proliferation and migration of malignant cells in vitro. These results highlight the critical role of viscous dissipation in the phenotype and proliferation of malignant cells, especially in stiff tumor environments.



中文翻译:

恶性细胞对粘性耗散的弹性依赖反应。

细胞环境的僵硬控制恶性细胞表型和增殖。然而,粘性耗散对这些参数的影响尚未得到研究,部分原因是缺乏可再现正常组织和肿瘤机械特性的体外细胞基质。在本文中,我们使用新报道的粘弹性聚丙烯酰胺凝胶细胞基质,并表征了粘性耗散对三种恶性细胞系的影响:来自前列腺的 DU145 和 PC3 和来自大脑的 LN229。在 1 kPa 和 5 kPa 弹性和粘弹性凝胶上分析这些细胞的扩散、运动和增殖率。令人惊讶的是,基材粘性耗散对细胞行为的影响取决于所测试的三种细胞类型的基材刚度。我们得出结论,粘弹性控制体外恶性细胞的扩散、增殖和迁移。这些结果强调了粘性耗散在恶性细胞的表型和增殖中的关键作用,尤其是在僵硬的肿瘤环境中。

更新日期:2020-08-12
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