Photosynthesis is one of the most important biological reactions and forms the basis of crop productivity and yield on which a growing global population relies. However, to develop improved plant cultivars that are capable of increased productivity, methods that can accurately and quickly quantify photosynthetic efficiency in large numbers of genotypes under field conditions are needed. Chlorophyll fluorescence imaging is a rapid, non-destructive measurement that can provide insight into the efficiency of the light-dependent reactions of photosynthesis. To test and validate a field-deployed fluorescence imaging system on the TERRA-REF field scanalyzer, leaves of potted sorghum plants were treated with a photosystem II inhibitor, DCMU, to reduce photochemical efficiency (FV/FM). The ability of the fluorescence imaging system to detect changes in fluorescence was determined by comparing the image-derived values with a handheld fluorometer. This study demonstrated that the imaging system was able to accurately measure photochemical efficiency (FV/FM) and was highly correlated (r = 0.92) with the handheld fluorometer values. Additionally, the fluorescence imaging system was able to track the decrease in photochemical efficiency due to treatment of DCMU over a 7 day period. The system’s ability to capture the temporal dynamics of the plants’ response to this induced stress, which has comparable dynamics to abiotic and biotic stressors found in field environments, indicates the system is operating correctly. With the validation of the fluorescence imaging system, physiological and genetic studies can be undertaken that leverage the fluorescence imaging capabilities and throughput of the field scanalyzer.

中文翻译：

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叶绿素荧光成像在田间环境中捕获谷物高粱（Sorghum bicolor）的光化学效率。

光合作用是最重要的生物反应之一，是全球人口不断增长所依赖的作物生产力和产量的基础。然而，为了开发能够提高生产力的改良植物品种，需要能够在田间条件下准确快速地量化大量基因型的光合效率的方法。叶绿素荧光成像是一种快速、无损的测量方法，可以深入了解光合作用的光依赖性反应的效率。为了在 TERRA-REF 现场扫描仪上测试和验证现场部署的荧光成像系统，用光系统 II 抑制剂 DCMU 处理盆栽高粱植物的叶子，以降低光化学效率 (FV/FM)。通过将图像衍生值与手持式荧光计进行比较来确定荧光成像系统检测荧光变化的能力。这项研究表明，成像系统能够准确测量光化学效率 (FV/FM)，并且与手持式荧光计值高度相关 (r = 0.92)。此外，荧光成像系统能够跟踪由于 DCMU 治疗超过 7 天而导致的光化学效率下降。该系统能够捕捉植物对这种诱导应激反应的时间动态，其动态与田间环境中发现的非生物和生物应激源具有可比性，这表明该系统运行正常。随着荧光成像系统的验证，