Objective: Nonthermal plasma at atmospheric condition using dielectric barrier discharge (DBD) is reported to be useful in many applications. Here, we have developed a strategy to generate nonthermal atmospheric plasma (NTAP) at ambient conditions for potential biomedical applications. Methods: We have explored the active ingredients of the nonthermal plasma using atomic emission spectroscopy. The potential mechanism of the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) from the generated plasma for therapeutic use has been demonstrated. Major results: The antimicrobial efficacy of the nonthermal plasma application to model Staphylococcus aureus and Candida albicans biofilms has been investigated. Our detailed electron microscopic studies followed by biochemical investigation reveal the mechanism of bacterial/fungal deactivation process. The nuclear magnetic resonance (NMR) studies on the live cell of C. albicans before and after NTAP treatment clearly conclude the disruption of cellular envelope leading to necrosis as evidenced by fluorescence-assisted cell sorting (FACS) studies. Conclusions: The proposed NTAP setup may find relevance in novel strategies in bacterial and fungal biofilm destruction in the future development of nonfluid hand sanitization.

中文翻译：

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非热大气等离子体诱导的金黄色葡萄球菌和白色念珠菌生物膜的细胞包膜损伤：光谱和生化研究

目标：据报道，在大气条件下使用介质阻挡放电 (DBD) 的非热等离子体可用于许多应用。在这里，我们开发了一种在环境条件下产生非热大气等离子体 (NTAP) 的策略，以用于潜在的生物医学应用。方法：我们使用原子发射光谱探索了非热等离子体的活性成分。从用于治疗的等离子体中产生活性氧 (ROS) 和活性氮 (RNS) 的潜在机制已经得到证实。主要结果：研究了非热等离子体应用对金黄色葡萄球菌和白色念珠菌生物膜模型的抗菌功效。我们详细的电子显微镜研究和生化研究揭示了细菌/真菌失活过程的机制。NTAP 治疗前后对白色念珠菌活细胞的核磁共振 (NMR) 研究清楚地得出结论，如荧光辅助细胞分选 (FACS) 研究所证明的那样，细胞包膜被破坏导致坏死。结论：提议的 NTAP 设置可能与非流体手部消毒未来发展中细菌和真菌生物膜破坏的新策略相关。