Mixed glial cells in primary cultures were exposed to amitriptyline (AT) and diazepam (Dia). Morphological observations during 17–18 days of exposure, revealed that 10μM and 100μM were critical concentrations for AT and Dia, respectively. Exposure to concentrations above the critical concentrations led to culture death. Concentrations below 10μM AT and 100μM Dia stimulated the proliferation of glial cells. The microglia cells were stimulated to proliferate and individual cells to hypertrophy. On the other hand, in Dia-treated cultures, the development of microglia cells did not differ from that in control cultures. Both drugs stimulated the development of oligodendroglia cells (OG). With decreasing concentrations of the drugs, the number of OG increased and the enzymes 2'3’-cyclic nucleotide 3’-phosphodiesterase (CNP) and acid phosphatase (AcP), were elevated in inverse proportion to the concentration of drugs.

中文翻译：

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原代培养物中神经胶质细胞重复暴露于阿米替林和地西泮

原代培养物中的混合神经胶质细胞暴露于阿米替林 (AT) 和地西泮 (Dia)。暴露 17-18 天期间的形态学观察表明，10μM 和 100μM 分别是 AT 和 Dia 的临界浓度。暴露于高于临界浓度的浓度会导致培养物死亡。低于 10μM AT 和 100μM Dia 的浓度会刺激神经胶质细胞的增殖。刺激小胶质细胞增殖和单个细胞肥大。另一方面，在 Dia 处理的培养物中，小胶质细胞的发育与对照培养物中的无差异。这两种药物都刺激了少突胶质细胞 (OG) 的发育。随着药物浓度的降低，OG 的数量增加，酶 2'