A method for generating targeted, pattern-generating, protein surface sensors via the self-assembly of modified oligodeox-ynucleotides (ODNs) is described. The simplicity by which these systems can be created enabled the development of a sen-sor that can straightforwardly discriminate between distinct glycoform populations. By using this sensor to identify glycosyl-ation states of a therapeutic protein, we demonstrate the diagnostic potential of this approach as well as the feasibility of integrating a wealth of supramolecular receptors and sensors into higher-order molecular analytical devices with advanced properties. For example, the facile device integration was used to attach the well-known anthracene-boronic acid (An-BA) probe to a biomimetic DNA scaffold and consequently, to use the unique photo-physical properties of An-BA to improve glycoform differentiation. In addition, the non-covalent assembly enabled us to modify the sensor with a tri-nitrilotriacetic acid (tri-NTA)-Ni2+ complex, which endows it with selectivity towards a hexa-histidine tag (His-tag). The selective responses of the system to diverse His-tag-labelled proteins further demonstrates the potential applicability of such sensors, as well as validates the mechanism underlying their function.

中文翻译：

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通过靶向、自组装、产生模式的蛋白质表面传感器进行糖型分化

描述了一种通过修饰寡脱氧核苷酸 (ODN) 的自组装生成有针对性的、生成模式的蛋白质表面传感器的方法。创建这些系统的简单性使得开发出一种可以直接区分不同糖型群体的传感器成为可能。通过使用这种传感器来识别治疗性蛋白质的糖基化状态，我们证明了这种方法的诊断潜力以及将大量超分子受体和传感器集成到具有先进特性的高阶分子分析设备中的可行性。例如，简单的设备集成用于将众所周知的蒽硼酸 (An-BA) 探针连接到仿生 DNA 支架上，因此，利用 An-BA 独特的光物理特性来改善糖型分化。此外，非共价组装使我们能够用三氮基三乙酸 (tri-NTA)-Ni2+ 复合物修饰传感器，使其具有对六组氨酸标签 (His-tag) 的选择性。系统对不同 His 标签标记的蛋白质的选择性反应进一步证明了此类传感器的潜在适用性，并验证了其功能背后的机制。