Percutaneous devices like orthopedic prosthetic implants for amputees, catheters, and dental implants suffer from high infection rates. A critical aspect mediating peri-implant infection of dental implants is the lack of a structural barrier between the soft tissue and the implant surface which could impede bacteria access and colonization of exposed implant surfaces. Parafunctional soft tissue regeneration around dental implants is marked by a lack of hemidesmosome formation and thereby weakened mechanical attachment. In response to this healthcare burden, a simultaneously hemidesmosome-inducing, antimicrobial, multifunctional implant surface was engineered. A designer antimicrobial peptide, GL13K, and a laminin-derived peptide, LamLG3, were coimmobilized with two different surface fractional areas. The coimmobilized peptide surfaces showed antibiofilm activity against Streptococcus gordonii while enhancing proliferation, hemidesmosome formation, and mechanical attachment of orally derived keratinocytes. Notably, the coatings demonstrated specific activation of keratinocytes: the coatings showed no effects on gingival fibroblasts which are known to impede the quality of soft tissue attachment to dental implants. These coatings demonstrated stability and retained activity against mechanical and thermochemical challenges, suggesting their intraoral durability. Overall, these multifunctional surfaces may be able to reduce peri-implantitis rates and enhance the success rates of all percutaneous devices via strong antimicrobial activity and enhanced soft tissue attachment to implants.

中文翻译：

---

用于半桥粒上调和预防细菌定植的角质形成细胞特异性肽基表面。

用于截肢者的骨科假肢植入物、导管和牙科植入物等经皮设备感染率很高。介导牙种植体种植体周围感染的一个关键方面是软组织和种植体表面之间缺乏结构屏障，这可能会阻碍细菌进入和暴露的种植体表面定植。牙种植体周围的辅助功能软组织再生的特点是缺乏半桥粒形成，从而削弱了机械附着力。为了应对这种医疗负担，设计了一种同时诱导半桥粒、抗菌、多功能的植入物表面。设计师抗菌肽 GL13K 和层粘连蛋白衍生肽 LamLG3 与两个不同的表面部分区域共固定。Streptococcus gordonii，同时增强口腔衍生角质形成细胞的增殖、半桥粒形成和机械附着。值得注意的是，涂层表现出对角质形成细胞的特异性激活：涂层对牙龈成纤维细胞没有影响，已知牙龈成纤维细胞会阻碍软组织附着在牙种植体上的质量。这些涂层表现出稳定性并保持对机械和热化学挑战的活性，表明它们的口腔内耐久性。总的来说，这些多功能表面可能能够通过强大的抗菌活性和增强的软组织与植入物的附着来降低种植体周围炎的发生率并提高所有经皮装置的成功率。