Untreated industrial waste affects fertility of agricultural soils. Among physical, chemical and biological methods, biological is the most efficient method to control pollution from soils affected with heavy metals. Experiments were conducted to assess role of strain MAI4 for adsorption and transformation and defense mechanism activation against metals as a mechanism of detoxification and their effect in augmenting bean growth. Strain MAI4 was identified as Pseudomonas sp. by 16 sRNA gene analysis. Maximum biosorption of metals by MAI4 was found at pH 6–8, 100–400 µg/ml and 24 h. Bioinoculant could reduce maximum amount of Cr (VI) at pH 6–8, 100–400 µg/ml and 15–35° C. Bioinoculant was able to form more biofilm under metal stress which suggested its detoxification role. Exopolysaccharides and antioxidants by strain MAI4 played a protective role against cell damage as metals could not release environmental deoxyribose nucleic acid (eDNA), superoxide and malondialdehyde (MDA). Protective role of strain MAI4 was also observed under scanning electron microscopy (SEM) and energy dispersive X- ray (EDX) spectroscopy. Strain prevented uptake of metals instead helped plants accumulate more nutrients in metal amended soil analyzed under energy dispersive X- ray (EDX) spectroscopy. Activation of defense mechanism, biosorption, biotransformation together with nutrient accumulation by strain MAI4 was responsible for growth promotion of beans.

未经处理的工业废物会影响农业土壤的肥力。在物理，化学和生物方法中，生物是控制重金属污染土壤污染的最有效方法。进行了实验以评估菌株MAI4在金属的吸附和转化以及防御机制激活（作为排毒机制）方面的作用及其在促进豆类生长中的作用。菌株MAI4被鉴定为假单胞菌sp。通过16 sRNA基因分析。在pH 6-8、100-400 µg / ml和24 h下，MAI4对金属的最大生物吸附作用。在pH为6-8、100-400 µg / ml和15-35°C的温度下，生物菌剂可以减少最大量的Cr（VI）。在金属胁迫下，生物菌剂能够形成更多的生物膜，这表明它具有排毒作用。MAI4菌株的胞外多糖和抗氧化剂起着防止细胞损伤的保护作用，因为金属不能释放环境中的脱氧核糖核酸（eDNA），超氧化物和丙二醛（MDA）。在扫描电子显微镜（SEM）和能量色散X射线（EDX）光谱下也观察到了菌株MAI4的保护作用。应变阻止了金属的吸收，反而帮助植物在能量色散X射线（EDX）光谱分析下在金属改良土壤中积累了更多的养分。激活防御机制，