The aim of the study was to determine the effects of inorganic (ferrous sulfate [FeSO₄] and FeSO₄ + phytase [FeSO₄+F]) and organic forms of iron (ferrous glycine chelate [FeGly], Fe-Gly + phytase [Fe-Gly+F]) on the concentrations of C-reactive protein (CRP), serum amyloid A (SA), alpha- 1-acid glycoprotein (α-AGP), haptoglobin (Hp), transferrin (TRF) and iron in the blood and liver tissue of poultry. Ross 308 roosters were used in this study. The acute-phase protein (AP) levels in the chicken serum and liver tissues were assayed using the double-antibody sandwich ELISA kits for chickens. The iron levels in serum and liver samples were measured using flame atomic absorption spectrophotometry. The results showed that the serum and hepatic iron concentration in the chickens receiving Fe-Gly and Fe-Gly-F were significantly higher than those in the control group and in the chickens receiving iron in the form of sulphates. Further, serum CRP and SA concentrations were lower in the chickens from the supplemented groups than in those from the control. The hepatic CRP concentration was higher in the supplemented groups than in the control group. The serum and hepatic TRF concentration were highest in the chickens receiving FeSO₄ and FeSO₄-F. The hepatic Hp concentration was higher in the chickens receiving organic forms of iron than in the control chickens, whereas the serum Hp concentrations were similar across the groups. The highest α-1-AGP concentration was found in the livers of the chickens receiving Fe- Gly and Fe-Gly-F. Taken together, these findings suggest that iron had the most beneficial effects for poultry in terms of health, performance and immunity when added to the feed in the form of glycine chelate.

中文翻译：

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日粮补充硫酸铁或甘氨酸螯合物对肉鸡血清和肝组织中急性期蛋白质和铁的生产性能和浓度的影响

该研究的目的是确定无机物（硫酸亚铁[FeSO ₄ ]和FeSO ₄ +植酸酶[FeSO ₄ + F]）和有机形式的铁（甘氨酸亚铁螯合物[FeGly]，Fe-Gly +植酸酶[ Fe-Gly + F]）上C反应蛋白（CRP），血清淀粉样蛋白A（SA），α-家禽血液和肝脏组织中的1-酸糖蛋白（α-AGP），触珠蛋白（Hp），转铁蛋白（TRF）和铁。这项研究使用了罗斯308头公鸡。使用用于鸡的双抗体夹心ELISA试剂盒测定了鸡血清和肝组织中的急性期蛋白（AP）水平。使用火焰原子吸收分光光度法测量血清和肝脏样品中的铁含量。结果表明，接受Fe-Gly和Fe-Gly-F的鸡的血清和肝铁浓度显着高于对照组和接受硫酸盐形式的铁的鸡。此外，补充组鸡的血清CRP和SA浓度低于对照组。补充组的肝CRP浓度高于对照组。接受FeSO的鸡的血清和肝TRF浓度最高₄和FeSO ₄ -F。接受有机形式铁的鸡的肝Hp浓度高于对照鸡，而各组的血清Hp浓度相似。在接受Fe-Gly和Fe-Gly-F的鸡的肝脏中发现了最高的α-1-AGP浓度。综上所述，这些发现表明，当以甘氨酸螯合物形式添加到饲料中时，铁对家禽的健康，性能和免疫力具有最有益的作用。