Cystic endometrial hyperplasia-pyometra complex (CEH/P) significantly perturbs the reproductive performance of affected bitches and ovariohysterectomy (OHE) is a commonly applied treatment. Thus the only way to take advantage of the genetic potential of valuable females is application of assisted reproductive techniques (ART) mainly in vitro embryo production (IVP) or in some exceptional cases animal cloning by somatic cell nuclear transfer (SCNT). The aim of our study was to examine a potential effect of the CEH/P status on the quality of oocytes from females subjected to OHE. In total, 828 immature oocytes collected from ovaries of 33 bitches (21 control, 12 CEH/P) were subjected to genetic analyses (mRNA expression of two maternal-effect genes: GDF-9, OCT4 and mitochondrial DNA (mtDNA) content). Oocytes of CEH/P females were characterized by a higher mtDNA content (471 696) than gametes of their healthy counterparts (368 175; P<0.005). Transcripts for the two genes were detected in all samples and the mRNA level was not affected by the CEH/P status. In conclusion, the CEH/P complex does not exert a negative effect on oocyte quality reflected by the two parameters examined in this study.

中文翻译：

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Pyometra不会影响犬卵母细胞的某些与分子质量相关的参数

囊性子宫内膜增生-脓疱复合物（CEH / P）明显干扰了受影响母犬的生殖性能，卵巢子宫切除术（OHE）是一种常用的治疗方法。因此，利用有价值女性的遗传潜能的唯一途径是应用辅助生殖技术（ART），主要是体外胚胎生产（IVP）或在某些特殊情况下通过体细胞核移植（SCNT）进行动物克隆。我们研究的目的是检验CEH / P状况对接受OHE的雌性卵母细胞质量的潜在影响。总共对从33个雌性卵巢（21个对照，12个CEH / P）的卵巢中收集的828个未成熟卵母细胞进行了遗传分析（两个母体效应基因GDF-9，OCT4的mRNA表达）和线粒体DNA（mtDNA）含量）。CEH / P雌性的卵母细胞的特征在于其mtDNA含量（471 696）高于其健康对应者的配子（368 175； P <0.005）。在所有样品中均检测到两个基因的转录本，mRNA水平不受CEH / P状态的影响。总之，CEH / P复合物不会对卵母细胞质量产生负面影响，这是由本研究中考察的两个参数反映出来的。