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Effect of dietary sodium acetate on skin mucus immune parameters and expression of gene related to growth, immunity and antioxidant system in common carp (Cyprinus carpio) intestine
Annals of Animal Science ( IF 1.9 ) Pub Date : 2020-08-10 , DOI: 10.2478/aoas-2020-0063
Roghieh Safari 1 , Seyed Hossein Hoseinifar 1 , Maryam Dadar 2 , Shabnam Nejadmoghaddam 1 , Hien Van Doan 3, 4
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The present study investigated the possible effects of including salt of short chain fatty acid, sodium acetate (SA), on skin mucus immune parameters and immune, antioxidant and growth-related genes expression in common carp. There is a little data available about the effective role of SA on immune, antioxidant and growth related genes expression as well as skin mucus immune parameters. The aim of this study was to analysis the effect of SA intake on these factors using common carp (Cyprinus carpio) as model organism. Two hundred and forty healthy common carp (mean weight = 15 ± 0.9 g) supplied and randomly stocked into 12 fiberglass tanks 200 L (20 fish per tank) assigned to four treatments and triplicates. The study was performed in a completely randomized design. The treatments were feeding carps with experimental diets containing different levels (0.0 [control], 0.5, 1 and 2%) of SA. The skin mucus total immunoglobulin and total protein levels in fish fed 2% SA showed significant increase compared to the control group (P < 0.05). Results showed a significant increase in the GH gene expression in 1 and 2% SA treatments (P < 0.05). The carps were fed with diet content 2% SA showed significantly increase in IGF-1 expression (P < 0.05). The expression of GSTa, and GPX (antioxidant genes) revealed a significant increase in the GSTa (fish fed SA at 1% and 2% levels) and GPX gene expression with fish fed 2% SA (P < 0.05). Supplementation of fish diet with SA induce slight elevation in the intestine of all immune-related genes (TNF-α, IL-1β, IL8 and Lyz) compared to the control group (P > 0.05). However, Lyz gene significantly up-regulated in 1 or 2% SA treatments. These results confirmed beneficial effects of SA as a feed additive in common carp culture.

中文翻译:

日粮乙酸钠对鲤鱼肠黏液免疫参数及与生长,免疫和抗氧化系统相关基因表达的影响

本研究调查了短链脂肪酸盐,醋酸钠(SA)可能对鲤鱼皮肤粘液免疫参数以及免疫,抗氧化剂和生长相关基因表达的影响。关于SA对免疫,抗氧化剂和生长相关基因表达以及皮肤粘液免疫参数的有效作用的可用数据很少。这项研究的目的是分析使用鲤鱼(鲤鱼)摄入SA对这些因素的影响。)作为模型有机体。供应了240头健康的鲤鱼(平均体重= 15±0.9克),并随机放入12个200升的玻璃纤维池中(每池20条鱼​​),分四次进行三次处理。该研究以完全随机的设计进行。治疗方法是给鲤鱼饲喂含有不同水平(0.0 [对照组],0.5、1和2%)SA的实验饮食。与对照组相比,饲喂2%SA的鱼的皮肤粘液总免疫球蛋白和总蛋白水平显着增加(P <0.05)。结果显示,在1%和2%的SA处理中,GH基因表达显着增加(P <0.05)。饲喂日粮含量为2%SA的鲤鱼显示IGF-1表达显着增加(P<0.05)。GSTa和GPX(抗氧化基因)的表达显示GSTa(以1%和2%的水平饲喂SA的鱼)和以2%的SA饲喂的鱼的GPX基因表达显着增加(P <0.05)。与对照组相比,鱼饲料中添加SA可诱导所有免疫相关基因(TNF-α,IL-1β,IL8和Lyz)的肠道轻度升高(P > 0.05)。但是,Lyz基因在1%或2%的SA处理中显着上调。这些结果证实了SA作为鲤鱼养殖的饲料添加剂的有益作用。
更新日期:2020-08-11
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